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Merck
CN

07-909

Anti-phospho-Src (Tyr418) 抗体

Upstate®, from rabbit

别名:

c-Src, p60-Src Src kinase

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
chicken, human, mouse
Application:
ICC, IHC, WB
Citations:
19
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biological source

rabbit

Quality Level

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

chicken, human, mouse

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr418)

Gene Information

human ... SRC(6714)

General description

60 kDa

Immunogen

Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)

Application

Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.
Research Category
Signaling
Research Sub Category
Cytoskeletal Signaling

Biochem/physiol Actions

Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.
Recognizes Src phosphorylated on Tyrosine residue 418.

Physical form

ImmunoAffinity Purified
Affinity Purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Routinely evaluated by immunoblot.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable



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Shinkyu Choi et al.
Antioxidants & redox signaling, 30(4), 505-519 (2018-01-18)
Altered redox state has been related to the development of normal pregnancy (NP) and preeclampsia (PE). Endothelial KCa2.3 and KCa3.1 (KCas) play an important role in vasodilation, and KCas levels are affected by oxidative stress. We investigated the mechanisms of
c-Src and cooperating partners in human cancer
Ishizawar, Rumey and Parsons, Sarah J
Cancer Cell, 6, 209-214 (2004)
Aida Habibovic et al.
JCI insight, 1(18), e88811-e88811 (2016-11-05)
Chronic inflammation with mucous metaplasia and airway remodeling are hallmarks of allergic asthma, and these outcomes have been associated with enhanced expression and activation of EGFR signaling. Here, we demonstrate enhanced expression of EGFR ligands such as amphiregulin as well



全球贸易项目编号

货号GTIN
07-90904053252335075