60 kDa

Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)

Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.

Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.

Recognizes Src phosphorylated on Tyrosine residue 418.

ImmunoAffinity Purified

Affinity Purified rabbit polyclonal IgG in PBS (without Mg²⁺ and Ca²⁺), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Control
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein

Routinely evaluated by immunoblot.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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