09-818
抗-CARM1抗体
from rabbit
别名:
Protein arginine N-methyltransferase 4, coactivator-associated arginine methyltransferase 1, coactivator-associated arginine methyltransferase-1
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
western blot
Species reactivity:
rat, human
Citations:
18
Technique(s):
western blot: suitable
Uniprot accession no.:
产品名称
抗-CARM1抗体, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, human
species reactivity (predicted by homology)
mouse
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CARM1(10498)
mouse ... Carm1(59035)
Analysis Note
对照
包括阳性抗原对照：目录号12-309，HeLa核提取物。将等体积的Laemmli样品还原性缓冲液加至10 μL裂解液中，煮沸5分钟以对制剂进行还原。 在微凝胶每个泳道上样20 μg经还原的裂解液。
包括阳性抗原对照：目录号12-309，HeLa核提取物。将等体积的Laemmli样品还原性缓冲液加至10 μL裂解液中，煮沸5分钟以对制剂进行还原。 在微凝胶每个泳道上样20 μg经还原的裂解液。
Application
抗CARM1抗体是一种兔多克隆抗体，用于检测CARM1（也称为蛋白质精氨酸N-甲基转移酶4，共激活因子相关的精氨酸甲基转移酶1），&已在WB中得到验证。
蛋白质印迹分析：
该批次的1 μg/mL稀释液在HeLa核提取物中检测到CARM1。（图A）。
该批次的1 μg/mL稀释液在HeLa核提取物中检测到CARM1。（图A）。
Biochem/physiol Actions
可识别CARM1，Mr ~60 kDa。
General description
CARM1（与共激活因子相关的精氨酸甲基转移酶1）是一种精氨酸N-甲基转移酶，可催化甲基从S-腺苷-L-蛋氨酸转移至蛋白质中的精氨酸残基。 CARM1目标包括Arg17处的组蛋白H3和Arg2142处的p300甲基化。基因激活后，CARM1与来自p300和p160家族的组蛋白乙酰转移酶一起被募集到启动子。在单核细胞炎症刺激过程中，CARM1与p300一起充当NF-kB的共激活因子。它负责在发育中的胚泡中将细胞移向内部细胞团。CARM1在雄激素受体中也起着重要作用，并可能在前列腺癌进展中起作用。
~60 kDa
Immunogen
含有序列（CSPMSIPTNTMHYGS）的KLH偶联的合成肽，对应于人CARM1的氨基酸595-608。
表位：氨基酸595-608
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
通过肽亲和层析纯化。在0.1M Tris-甘氨酸（pH 7.4），15 mM NaCl和0.05% 叠氮化钠中以1 mg/ml提供。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Heng Hong et al.
Cancer, 101(1), 83-89 (2004-06-29)
Coactivator-associated arginine methyltransferase 1 (CARM1) is a transcriptional coactivator of the androgen receptor (AR). It is involved in the regulation of the biologic functions of the AR. It remains to be determined whether CARM1 is involved in prostatic carcinogenesis. The
Maria-Elena Torres-Padilla et al.
Nature, 445(7124), 214-218 (2007-01-12)
It has been generally accepted that the mammalian embryo starts its development with all cells identical, and only when inside and outside cells form do differences between cells first emerge. However, recent findings show that cells in the mouse embryo
D Chen et al.
Science (New York, N.Y.), 284(5423), 2174-2177 (1999-06-26)
The p160 family of coactivators, SRC-1, GRIP1/TIF2, and p/CIP, mediate transcriptional activation by nuclear hormone receptors. Coactivator-associated arginine methyltransferase 1 (CARM1), a previously unidentified protein that binds to the carboxyl-terminal region of p160 coactivators, enhanced transcriptional activation by nuclear receptors
Cynthia M Quintero et al.
Journal of molecular biology, 430(21), 4168-4182 (2018-08-29)
Activation of the retinoic acid (RA) signaling pathway is important for controlling embryonic stem cell differentiation and development. Modulation of this pathway occurs through the recruitment of different epigenetic regulators at the retinoic acid receptors (RARs) located at RA-responsive elements
The expression of myogenic microRNAs indirectly requires protein arginine methyltransferase (Prmt)5 but directly requires Prmt4.
Mallappa, C; Hu, YJ; Shamulailatpam, P; Tae, S; Sif, S; Imbalzano, AN
Nucleic Acids Research null
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