1.01167
Amido black 10 B (C.I.20470)
for electrophoresis
别名:
Amido black 10 B (C.I.20470), Black acid 1, Amido black 10 B (C.I. 20470), Naphthol blue black
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关于此项目
经验公式（希尔记法）:
C22H14N6Na2O9S2
化学文摘社编号:
分子量:
616.49
EC Index Number:
213-903-1
UNSPSC Code:
41105305
MDL number:
Quality Segment
form
solid
potency
>5000 mg/kg LD50, oral (Rat)
composition
Dye, ≥80% spectrophotometric assay
pH
9.0 (25 °C, 10 g/L in H2O)
solubility
10 g/L
bulk density
670 kg/m3
εmax
750-960 at 617-620 nm in water at 0.01 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
InChI
1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,24H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25?,27-21+;;
InChI key
HKBVRFLHNUEVRO-DWTBGCDMSA-L
General description
Amido black 10 B (C.I. 20470) for electrophoresis, is used for staining connective tissue, in electrophoresis, and as a redox indicator. Proteins possess free amino and carboxyl groups and are charged positively or negatively in aqueous solutions, depending on the pH. In an alkaline milieu they are negatively charged and migrate in the electric field towards the anode at a speed that depends on the number of charges they carry. This difference in migration velocities makes it possible to separate the serum protein mixture into groups of individual components.The separated protein fractions are stained with amido black and measured photometrically or densitometrically. As a rule, five fractions are observed after staining - albumin, alpha 1- and alpha 2-globulins, beta 1- and beta 2-globulins, and beta-globulins - where albumin has the highest migration velocity and gamma-globulin the lowest. Differentiation of serum proteins by electrophoresis is important for the diagnosis and prognosis of numerous diseases. Changes in protein composition can be found e. g. in inflammatory diseases, tumors, liver diseases, antibody deficiency syndromes or disturbances in protein balance. As an IVD product and CE registered, it can be used hassle-free in diagnostics and laboratory accreditations. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Dye content (spectrophotometrically): ≥ 80 %
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 617 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water): 750 - 960
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for electrophoresis: passes test
Suitability as redox indicator: passes test
Identity
- sodium:
passes test
- UV/VIS-Spectrum: passes test
Absorption maximum λ max (water): 617 - 620 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.01 g/l; water): 750 - 960
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for electrophoresis: passes test
Suitability as redox indicator: passes test
存储类别
11 - Combustible Solids
flash_point_c
Not applicable
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1B
flash_point_f
Not applicable
wgk
WGK 1