Quality Segment
description
shipped with cooling packs
product line
Cellvento®
form
dry powder
technique(s)
cell culture | mammalian: suitable (suspension)
application(s)
pharma/biopharma processes
shipped in
ambient
storage temp.
2-8°C
General description
Cellvento® BHK-200 medium is a serum-free cell culture medium, formulated without any animal derived component and optimized for the culture of suspension BHK21 cells at high-density and viability and efficient propagation of viruses. BHK21 cells can be grown as suspension cultures in T-flasks, shaker flasks, spinner bottles or stirred tank bioreactors.
Application
- Cellvento® BHK-200 medium has been developed for BHK-21 cell line but is not limited to this application and may suit other vaccine producing cell lines as well.
- Qualified for instance for the production of Foot and Mouth Disease virus (FMDV).
- When the density of BHK21 cells satisfies process requirement, the virus can be inoculated directly to the reactor without media exchange or cell separation/ sedimentation steps, resulting in highly efficient virus production.
- It is recommended to use only 70% of the maximum bioreactor volume for cell growth. After cells have reached the desired cell density, 30% fresh medium can be added in the reactor before inoculation and propagation of the virus.
Features and Benefits
- Serum-free, animal-component-free formulation for improved process consistency.
- Chemically defined components to enable better batch-to-batch reproducibility and easier regulatory documentation.
- Optimized for compatibility with standard BHK cell lines and common feeds/additives used in upstream processing.
Preparation Note
Formulated to contain 21.6658 grams of powder per liter of medium.
Legal Information
CELLVENTO is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1