Fast green FCF (C.I. 42053)

The dry dye Fast Green FCF (C.I. 42053) for microscopy Certistain^®, is used for the preparation of a staining solution that can be used for modified connective tissue staining acc. to van Gieson in histological sections. Further it is applied in histology as counterstaining in e.g. trichrome staining acc. to Masson and additionally for histochemical staining, as well as in Cytology as counterstain instead of light green SF in Papanicolaou′s staining. As CERTISTAIN^® dye it is chemically quality-certified according to highest standards and strict specifications.

for microscopy Certistain^®

Dye content (spectrophotometrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λ_max; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany