InChI
1S/C37H36N2O10S3.2Na/c1-3-38(24-26-7-5-9-33(21-26)50(41,42)43)30-15-11-28(12-16-30)37(35-20-19-32(40)23-36(35)52(47,48)49)29-13-17-31(18-14-29)39(4-2)25-27-8-6-10-34(22-27)51(44,45)46;;/h5-23H,3-4,24-25H2,1-2H3,(H3-,40,41,42,43,44,45,46,47,48,49);;/q;2*+1/p-2
InChI key
XJBPDZVCYTYRIN-UHFFFAOYSA-L
form
solid
potency
>2000 mg/kg LD50, oral (Rat)
composition
Dye, ≥85% spectrophotometric assay
pH
2.7 (20 °C, 10 g/L in H2O)
mp
290 °C (decomposition)
solubility
150 g/L
bulk density
350 kg/m3
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
Quality Level
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Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysis Note
Dye content (spectrophotometrically): ≥ 85 %
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (ethanol 50 %): 622 - 626 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.003 g/l; ethanol 50 %): 1360 - 1610
TLC-Test: passes test
Loss on drying (110 °C): ≤ 10 %
Suitability for microscopy: passes test
Application
for microscopy Certistain®
General description
The dry dye Fast Green FCF (C.I. 42053) for microscopy Certistain®, is used for the preparation of a staining solution that can be used for modified connective tissue staining acc. to van Gieson in histological sections. Further it is applied in histology as counterstaining in e.g. trichrome staining acc. to Masson and additionally for histochemical staining, as well as in Cytology as counterstain instead of light green SF in Papanicolaou′s staining. As CERTISTAIN® dye it is chemically quality-certified according to highest standards and strict specifications.
signalword
Warning
hcodes
Hazard Classifications
Muta. 2 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
352.4 °F - closed cup
flash_point_c
178 °C - closed cup
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