Quality Segment
material
aluminum support, silica gel 60 matrix
feature
binder Organic Polymer, fluorescent indicator
packaging
pkg of 25 sheets
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
20 cm × 20 cm
sheet L × W
20 cm × 20 cm
particle size
10-12 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
General description
25 Aluminium sheets 20 x 20 cm
Precoated Kieselguhr or mixed Silica gel/Kieselguhr plates are only of small importance nowadays. Kieselguhr layers are used like cellulose layers for the TLC separation of polar and moderately polar substances. Polyamide, on the other hand , is used for the separation of flavonoids and other phenolic substances.
Analysis Note
Layer thickness: 170 - 220 µm
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testing:
amino acid test
hRf-values
- β-alanine, short-time test:
25 - 30
- L-alanine, amino acid test: 45 - 55
- DL-Valin, amino acid test: 75 - 85
- L-leucine, amino acid test: 85 - 95
Typical value determined on a sheet
Eluent: n- propanol/ water (80/20 v/v)
Deviation of layer thickness per plate: ≤ 30 µm
Chromatographic testing:
amino acid test
hRf-values
- β-alanine, short-time test:
25 - 30
- L-alanine, amino acid test: 45 - 55
- DL-Valin, amino acid test: 75 - 85
- L-leucine, amino acid test: 85 - 95
Typical value determined on a sheet
Eluent: n- propanol/ water (80/20 v/v)
Other Notes
Replaces: 5568-7; 5568