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Merck
CN

1.07393

蛋白酶 K (来源于真菌)，用于生物化学

(from Tritirachium album) solution in Tris/HCl pH 7.5; 0.01 mol/l; 600 mAnson-U/ml; for molecular biology EC 3.4.21.14

别名:

ProK, native proteinase K solution, non-specific protease

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NACRES:
NA.51
UNSPSC Code:
12352204

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form

liquid

pH

7.5 (25 °C in H2O, undiluted)

density

1.10 g/cm3 at 20 °C

storage temp.

2-8°C

Quality Level

400

General description

蛋白酶K是一种肽链内切酶，属于蛋白酶的枯草杆菌蛋白酶家族。多肽链含有278 个氨基酸和催化三联体Asp39、His69、Ser224。

Application

蛋白酶K已用于：
  • 免疫沉淀样品的去交联
  • 脱氧核糖核苷酸末端转移酶dUTP缺口末端标记（TUNEL）测定中的多聚-L-赖氨酸包被的结肠组织载玻片的处理
  • 原位杂交

Biochem/physiol Actions

蛋白酶 K 催化酯和肽键的水解。它和水化的非水溶剂一起用于合成肽。蛋白酶 K用于打破福尔马林固定产生的交联键，并使引物和聚合酶的靶序列暴露出来。

Analysis Note

外观（颜色）：无色
外观（清晰度）：透明
活性（血红蛋白；pH 7.5；37 °C）： ≥ 600 mAnsonU/ml
比活度（以蛋白质计）： ≥ 40.0 mAnsonU/mg
DNases（切割活性；pBR 322；6 h；37 °C）：无法检测
RNases（RNA；2 h；37°C）：无法检测
菌落数（需氧菌）： ≤ 10 CFU/ml

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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F Chavagnat et al.
Applied and environmental microbiology, 65(7), 3001-3007 (1999-07-02)
The general aminopeptidase PepN from Streptococcus thermophilus A was purified to protein homogeneity by hydroxyapatite, anion-exchange, and gel filtration chromatographies. The PepN enzyme was estimated to be a monomer of 95 kDa, with maximal activity on N-Lys-7-amino-4-methylcoumarin at pH 7
María Isabel Navarro-Mendoza et al.
Current biology : CB, 29(22), 3791-3802 (2019-11-05)
Centromeres are rapidly evolving across eukaryotes, despite performing a conserved function to ensure high-fidelity chromosome segregation. CENP-A chromatin is a hallmark of a functional centromere in most organisms. Due to its critical role in kinetochore architecture, the loss of CENP-A

商品

Best Product & Supplier for Proteinase K

Balancing Proteinase K cost with quality and technical support ensures optimal enzyme selection for diverse applications.

Guide on How to Use Proteinase K in Different Procedures

Guidelines on use of proteinase K, an enzyme commonly used to degrade proteins, and protect DNA and RNA from degradation in samples.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

Proteinase K: Introduction & Applications

Proteinase K aids in molecular biology applications by digesting structural proteins, removing nucleases, and isolating intact genomic DNA.

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