1.08218
三氟乙酸 溶液
(25% solution in water) for protein sequenation
别名:
TFA
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关于此项目
线性分子式:
CF3COOH
化学文摘社编号:
分子量:
114.02
NACRES:
NB.21
UNSPSC Code:
12352106
Beilstein/REAXYS Number:
5446934
MDL number:
Concentration:
24.5-25.5% (trifluoroacetic acid)
Form:
liquid
Quality Segment
form
liquid
composition
Water, 74.5-75.5% Karl Fischer
concentration
24.5-25.5% (trifluoroacetic acid)
density
1.12 g/cm3 at 20 °C
storage temp.
15-25°C
SMILES string
OC(=O)C(F)(F)F
InChI
1S/C2HF3O2/c3-2(4,5)1(6)7/h(H,6,7)
InChI key
DTQVDTLACAAQTR-UHFFFAOYSA-N
Application
Trifluoroacetic acid (TFA) can be used as a solvent in a reverse-phase HPLC system to separate peptides because it enhances peak resolution. It also has low absorption detection wavelengths and is relatively volatile and easily removed. For the majority of peptide separations, TFA has been used at concentrations ranging from 0.05 to 0.1%(6.5-13 mM).
Analysis Note
Assay (alkalimetric): 24.5 - 25.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
Identity (IR-spectrum): passes test
Density (d 20 °C/ 4 °C): 1.129 - 1.131
Fluorescence (at 254 nm): ≤ 2 ppb
Fluorescence (at 365 nm): ≤ 2 ppb
Water (according to Karl Fischer): 74.5 - 75.5 %
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警示词
Danger
supp_hazards
闪点 (F)
Not applicable
闪点 (°C)
Not applicable
危险代码
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Corr. 1A
存储类别
8B - Non-combustible corrosive hazardous materials