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Merck
CN

113200

Sigma-Aldrich

Anti-α-Actin Mouse mAb (1A4)

liquid, clone 1A4, Calbiochem®

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

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生物来源

mouse

质量水平

100

抗体产品类型

primary antibodies

克隆

1A4, monoclonal

表单

liquid

包含

≤0.1% sodium azide as preservative

种属反应性

baboon, rabbit, human

制造商/商品名称

Calbiochem®

储存条件

do not freeze

dilution

(Frozen Sections (1:50-1:75)
Immunoblotting
Paraffin Sections (1:50-1:75))

同位素/亚型

IgG2a

运输

wet ice

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... ACTA1(58)

相关类别

一般描述

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
    • This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    免疫原

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    应用

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    包装

    Please refer to vial label for lot-specific concentration.

    外形

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    分析说明

    Negative Control
    Fibroblasts, striated muscle, or myocardium
    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

    其他说明

    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    免责声明

    Toxicity: Standard Handling (A)

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    储存分类代码

    12 - Non Combustible Liquids

    WGK

    WGK 2

    闪点(°F)

    Not applicable

    闪点(°C)

    Not applicable

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