113200
Anti-α-Actin Mouse mAb (1A4)
liquid, clone 1A4, Calbiochem®
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Clone:
1A4, monoclonal
Species reactivity:
baboon, rabbit, human
Application:
Citations:
12
产品名称
Anti-α-Actin Mouse mAb (1A4), liquid, clone 1A4, Calbiochem®
isotype
IgG2a
biological source
mouse
antibody product type
primary antibodies
clone
1A4, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
baboon, rabbit, human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Frozen Sections (1:50-1:75)
Immunoblotting
Paraffin Sections (1:50-1:75))
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ACTA1(58)
Analysis Note
Negative Control
Fibroblasts, striated muscle, or myocardium
Fibroblasts, striated muscle, or myocardium
Positive Control
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
Application
Frozen Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Immunoblotting (see comments)
Paraffin Sections (1:50-1:75)
Disclaimer
Toxicity: Standard Handling (A)
General description
Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
- Antibody Target Gene Symbol: ACTA2
- Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
- Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
- Hu Entrez ID: 59 (Related Antibodies: 113200 )
- Rat Entrez ID: 81633
Immunogen
Human Smooth Muscle
a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.
Other Notes
Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Tobias Reicherzer et al.
American journal of physiology. Lung cellular and molecular physiology, 315(1), L87-L101 (2018-04-13)
Mesenchymal stromal cells (MSCs) are released into the airways of preterm infants following lung injury. These cells display a proinflammatory phenotype and are associated with development of severe bronchopulmonary dysplasia (BPD). We aimed to characterize the functional properties of MSCs
Aya Nomura-Kitabayashi et al.
Methods in molecular biology (Clifton, N.J.), 1816, 253-268 (2018-07-11)
We introduced the vascular remodeling mouse system induced by the wire injury to investigate the molecular and cellular mechanisms of cardiovascular diseases. Using these models, we focus on the adventitial cell population in the outermost layer of the adult vasculature
Tayyab Shahzad et al.
Cell death & disease, 13(7), 614-614 (2022-07-16)
The hyperoxia-induced pro-inflammatory response and tissue damage constitute pivotal steps leading to bronchopulmonary dysplasia (BPD) in the immature lung. The pro-inflammatory cytokines are considered attractive candidates for a directed intervention but the complex interplay between inflammatory and developmental signaling pathways
Tomohiro Ohtsuki et al.
Journal of the American Heart Association, 8(23), e013716-e013716 (2019-11-23)
Background Circulating proteins are exposed to vascular endothelial layer and influence their functions. Among them, adipsin is a member of the trypsin family of peptidases and is mainly secreted from adipocytes, monocytes, and macrophages, catalyzing the rate-limiting step of the
Xiaoliang Yan et al.
Experimental and therapeutic medicine, 23(4), 292-292 (2022-03-29)
The recently identified adipocytokine omentin was previously found to be expressed mainly in human omental and visceral adipose tissues. As such, reduced plasma concentrations of omentin were revealed to be associated with increased risks of cardiovascular diseases. Omentin has also
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