Merck
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113200

Sigma-Aldrich

Anti-α-Actin Mouse mAb (1A4)

liquid, clone 1A4, Calbiochem®

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生物来源

mouse

质量水平

100

antibody product type

primary antibodies

克隆

1A4, monoclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

species reactivity

baboon, rabbit, human

manufacturer/tradename

Calbiochem®

储存条件

do not freeze

同位素/亚型

IgG2a

运输

wet ice

储存温度

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ACTA1(58)

一般描述

Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
    • This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    免疫原

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    应用

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    包装

    Please refer to vial label for lot-specific concentration.

    警告

    Toxicity: Standard Handling (A)

    外形

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    分析说明

    Negative Control
    Fibroblasts, striated muscle, or myocardium
    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary

    其他说明

    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.
    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    储存分类代码

    12 - Non Combustible Liquids

    WGK

    WGK 2

    闪点(°F)

    Not applicable

    闪点(°C)

    Not applicable

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