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Merck
CN
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文件

1.15444

Sigma-Aldrich

考马斯亮蓝G250 (C.I.42655)

for electrophoresis

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别名:
Coomassie亮蓝 G 250 (C.I.42655), SERVA蓝G, 极地蓝G, 酸性蓝90, 青蓝G
经验公式(希尔记法):
C47H48N3NaO7S2
CAS号:
分子量:
854.02
MDL编号:
UNSPSC代码:
41105305
EC索引号:
228-058-4
NACRES:
NC.01

质量水平

效能

>5000 mg/kg LD50, oral (Rat)

技术

protein staining: suitable

pH值(酸碱度)

6.4 (20 °C, 10 g/L in H2O)

溶解性

40 g/L

堆积密度

520 kg/m3

储存温度

no temp limit

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key

CMGWKYXJIYYESN-UHFFFAOYSA-M

一般描述

Coomassie亮蓝G 250 (C.I.42655)是Coomassie亮蓝(CBB)之一,是一种三苯基甲烷染料。CBB染料广泛用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的蛋白质显色。CBB G-250(绿蓝色)染料适用于Bradford测定的蛋白定量。

应用

Coomassie亮蓝G 250 (C.I.42655)已用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的凝胶染色。它还用于Bradford蛋白测定。

分析说明

鉴定标准(UV/VIS-光谱):通过测试
最大吸光值λ最大(缓冲液pH7.0):577 - 584 nm
光谱吸光性A 1%/1cm(λ最大;0.01 g/l;缓冲液pH 7.0;基于干物质计算):450 - 570
TLC-测试:通过测试
干燥损失(100 °C): ≤ 8 %
电泳适用性:通过测试

法律信息

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Robert G E Krause et al.
Analytical biochemistry, 566, 107-115 (2018-11-21)
Coomassie brilliant blue R250, an anionic dye is the most popular stain to detect proteins resolved in SDS-PAGE gels. Crystal violet, a cationic dye was found to be versatile and stained proteins in SDS-PAGE gels and in zymograms. Stained proteins
Nazim Mekaoui et al.
Journal of chromatography. A, 1232, 134-141 (2011-12-22)
Commercial samples of Coomassie Brilliant Blue G-250 (CBB) were not pure enough to give reliable results when used as indicator of amine content in biological material. The polar and apolar impurities produce unacceptable biases in the results. Counter current chromatography
Gang Lu et al.
Bio-protocol, 10(6), e3565-e3565 (2021-03-05)
Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice
Mohammad-Bagher Ebrahim-Habibi et al.
Scientific reports, 9(1), 1273-1273 (2019-02-06)
Investigation of non-covalent interaction of hydrophobic surfaces with the protein G (PrG) is necessary due to their frequent utilization in immunosensors and ELISA. It has been confirmed that surfaces, including carbonous-nanostructures (CNS) could orient proteins for a better activation. Herein

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