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Merck
CN

1.15444

考马斯亮蓝G250 (C.I.42655)

for electrophoresis

别名:

Coomassie亮蓝 G 250 (C.I.42655), SERVA蓝G, 极地蓝G, 酸性蓝90, 青蓝G

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关于此项目

经验公式（希尔记法）:
C47H48N3NaO7S2
化学文摘社编号:
6104-58-1
分子量:
854.02
UNSPSC Code:
41105305
EC Index Number:
228-058-4
NACRES:
NC.01
MDL number:
MFCD00078482

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SMILES string

[Na+].[S](=O)(=O)([O-])c1cc(ccc1)CN(CC)c2cc(c(cc2)[C+](c5c(cc(cc5)N(CC)Cc6cc(ccc6)[S](=O)(=O)[O-])C)c3ccc(cc3)Nc4ccc(cc4)OCC)C

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key

CMGWKYXJIYYESN-UHFFFAOYSA-M

potency

>5000 mg/kg LD50, oral (Rat)

technique(s)

protein staining: suitable

pH

6.4 (20 °C, 10 g/L in H2O)

solubility

40 g/L

bulk density

520 kg/m3

storage temp.

no temp limit

Quality Level

100

相关类别

General description

Coomassie亮蓝G 250 (C.I.42655)是Coomassie亮蓝(CBB)之一，是一种三苯基甲烷染料。CBB染料广泛用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的蛋白质显色。CBB G-250（绿蓝色）染料适用于Bradford测定的蛋白定量。

Application

Coomassie亮蓝G 250 (C.I.42655)已用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的凝胶染色。它还用于Bradford蛋白测定。

Analysis Note

鉴定标准（UV/VIS-光谱）：通过测试
最大吸光值λ最大（缓冲液pH7.0）：577 - 584 nm
光谱吸光性A 1%/1cm（λ最大；0.01 g/l；缓冲液pH 7.0；基于干物质计算）：450 - 570
TLC-测试：通过测试
干燥损失（100 °C）： ≤ 8 %
电泳适用性：通过测试

Legal Information

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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Nazim Mekaoui et al.
Journal of chromatography. A, 1232, 134-141 (2011-12-22)
Commercial samples of Coomassie Brilliant Blue G-250 (CBB) were not pure enough to give reliable results when used as indicator of amine content in biological material. The polar and apolar impurities produce unacceptable biases in the results. Counter current chromatography
Robert G E Krause et al.
Analytical biochemistry, 566, 107-115 (2018-11-21)
Coomassie brilliant blue R250, an anionic dye is the most popular stain to detect proteins resolved in SDS-PAGE gels. Crystal violet, a cationic dye was found to be versatile and stained proteins in SDS-PAGE gels and in zymograms. Stained proteins
Gang Lu et al.
Bio-protocol, 10(6), e3565-e3565 (2021-03-05)
Although many spherical and rod-shaped plant virus purification protocols are now available, only a few protocols on filamentous plant virus purification have been published. Here, we report a protocol for large-scale purification of Rice stripe virus (RSV) from RSV-infected rice
Mohammad-Bagher Ebrahim-Habibi et al.
Scientific reports, 9(1), 1273-1273 (2019-02-06)
Investigation of non-covalent interaction of hydrophobic surfaces with the protein G (PrG) is necessary due to their frequent utilization in immunosensors and ELISA. It has been confirmed that surfaces, including carbonous-nanostructures (CNS) could orient proteins for a better activation. Herein

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