1.15940
Crystal violet (C.I. 42555)
for microscopy Certistain®
别名:
Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B
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关于此项目
经验公式（希尔记法）:
C25H30ClN3
化学文摘社编号:
分子量:
407.98
EC Index Number:
208-953-6
UNSPSC Code:
41116130
MDL number:
Quality Segment
form
solid
potency
420 mg/kg LD50, oral (Rat)
composition
Dye, ≥88% spectrophotometric assay
pH
2.5-3.5 (20 °C, 10 g/L in H2O)
mp
189-194 °C
solubility
10 g/L
density
1.19 g/cm3 at 20 °C
bulk density
220‑400 kg/m3
εmax
2050-2450 at 589-594 nm in water at 0.002 g/L
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-30°C
InChI
1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1
InChI key
ZXJXZNDDNMQXFV-UHFFFAOYSA-M
General description
Crystal violet (C.I. 42555) for microscopy Certistain®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Analysis Note
Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λmax; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test
Legal Information
CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany
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Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1
存储类别
11 - Combustible Solids
wgk
WGK 3