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Merck
CN

12-301

Non-Stimulated A431 Cell Lysate

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关于此项目

UNSPSC Code:
41105330
NACRES:
NA.41
eCl@ss:
32011807
Technique(s):
western blot: suitable
Species reactivity:
-
Application:
WB
Citations:
7
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biological source

human

Quality Level

form

liquid

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

shipped in

dry ice

Biochem/physiol Actions

A431

Physical form

modified RIPA buffer (50mM Tris-HCl, pH 7.4, 1% NP40, 0.25% sodium deoxycholate, 150mM NaCl, 1mM EDTA, 1mM PMSF, 1μg/ml each of aprotinin, leupeptin, pepstatin, 1mM Na3VO4, 1mM NaF) and diluted with non-reducing sample buffer (31mM Tris-HCl, pH 6.8, 5% glycerol, 1% SDS, 0.002% bromphenol blue).

Preparation Note

1 year at -20°C

Analysis Note

Routinely evaluated as a positive control for several of Upstates antibodies.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


pictograms

Exclamation mark

存储类别

12 - Non Combustible Liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2

法规信息

高风险级别生物产品-Merck

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D J Flint et al.
Journal of molecular endocrinology, 36(3), 435-448 (2006-05-25)
Insulin-like growth factor-binding protein 5 (IGFBP-5) mediates involution of the mammary gland. The decrease in DNA content and mammary gland weight which accompanies involution was inhibited by prolactin (PRL) in wild-type but not transgenic mice expressing IGFBP-5. Phospho-STAT5 protein levels
Norikazu Yabuta et al.
Scientific reports, 8(1), 4892-4892 (2018-03-22)
p63, a transcriptional factor that belongs to the p53 family, regulates epidermal differentiation, stemness, cell death, tumorigenesis, metastasis, and senescence. However, its molecular mechanism remains elusive. We report here that TAp63 phosphorylated at T46/T281 specifically upregulates the late cornified envelope
Johannes Witt et al.
BMC systems biology, 3, 71-71 (2009-07-18)
Biological effects of nuclear factor-kappaB (NF kappaB) can differ tremendously depending on the cellular context. For example, NF kappaB induced by interleukin-1 (IL-1) is converted from an inhibitor of death receptor induced apoptosis into a promoter of ultraviolet-B radiation (UVB)-induced



全球贸易项目编号

货号GTIN
12-30108436037123535
100961000104022536869223