assay
≥70% (Coomassie staining, SDS-PAGE)
form
liquid
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... PPP1R12A(4659)
Analysis Note
Routinely evaluated as a substrate in a Protein Kinase Assay.
Biochem/physiol Actions
Protein Target: myosin phosphatase
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Product Source: expressed in E. coli
ROK substrate protein corresponding to amino acids 654-880 of human MYPT1 with an N-terminal GST tag. MW = 60 kDa.
Physical form
100mM Tris-HCl pH 8.0, 200mM NaCl, 10mM glutathione and 5μg/ml leupeptin. Frozen solution.
Preparation Note
1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the vial prior to removing the cap.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
G Totsukawa et al.
The Journal of cell biology, 144(4), 735-744 (1999-02-26)
It has been demonstrated previously that during mitosis the sites of myosin phosphorylation are switched between the inhibitory sites, Ser 1/2, and the activation sites, Ser 19/Thr 18 (Yamakita, Y., S. Yamashiro, and F. Matsumura. 1994. J. Cell Biol. 124:129-
J Feng et al.
The Journal of biological chemistry, 274(52), 37385-37390 (1999-12-22)
It is clear from several studies that myosin phosphatase (MP) can be inhibited via a pathway that involves RhoA. However, the mechanism of inhibition is not established. These studies were carried out to test the hypothesis that Rho-kinase (Rho-associated kinase)
Yi-Ju Lee et al.
Journal of cellular physiology, 220(2), 476-484 (2009-04-25)
Cellular responses are determined by a number of signaling cues in the local microenvironment, such as growth factors and extracellular matrix (ECM). In cultures of mammary epithelial cells (MECs), functional differentiation requires at least two types of signal, lactogenic hormones
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