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Merck
CN

12-500

Sigma-Aldrich

HeLa + EGF Stimulated Cell Lysate in Mg2+ Lysis Buffer

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关于此项目

UNSPSC代码:
41105330
eCl@ss:
32011807
NACRES:
NA.42
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生物来源

human

质量水平

表单

liquid

制造商/商品名称

Upstate®

技术

western blot: suitable

运输

dry ice

一般描述

EGF stimulated HeLa cell lysate. The cell lysate was quantitated via BCA assay. This sell lysate demonstrated higher levels of Ras-GTP form present as compared to unstimulated HeLa cell lysates. The assay was performed using the Ras GTPase Activation ELISA Kit (Catalog # 17-424)

生化/生理作用

HeLa

外形

100 &micor;g of EGF stimulated HeLa cell lysate, in 40 µL of 1X MLB (Catalog 20-168) containing, 1 &micor;g/mL leupeptin, 1µg/mL aprotinin, 1 µg/mL pepstatin, 1mM PMSF.

制备说明

Stable for 6 months at -80C from date of shipment. For maximum recovery of product, centrifuge the vial prior to removing the cap. Use immediately in assay following thawing as Ras GTP form can rapidly hydrolyze to the inactive Ras GDP form.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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