14-173-M
MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.
登录 查看组织和合同定价。
选择尺寸
关于此项目
UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
mouse
Recombinant:
expressed in E. coli
产品名称
MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg, Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.
biological source
mouse
recombinant
expressed in E. coli
mol wt
Mw 67.8 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Quality Level
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Preparation Note
Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.
Analysis Note
Routinely evaluated by phosphorylation myelin basic protein
Biochem/physiol Actions
Protein Target: MAPK2
Target Sub-Family: CMGC
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Packaging
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.
Physical form
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Glutathione agarose; Ni2+/NTA agarose
signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
10 - Combustible liquids
wgk
WGK 2
Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
Sanghera, J S, et al.
The Journal of Biological Chemistry, 265, 52-57 (1990)
X Yang et al.
The Journal of biological chemistry, 273(45), 29879-29887 (1998-10-29)
The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in virus replication and infectivity. Here we show that Vif is phosphorylated and regulated by p44/42 mitogen-activated protein kinase (MAPK). Vif phosphorylation by MAPK was demonstrated in
Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
Sanghera, J S, et al.
Biochimica et Biophysica Acta, 1095, 153-160 (1991)
Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
Sutherland, C, et al.
European Journal of Biochemistry, 212, 581-588 (1993)
C Zhang et al.
The Journal of biological chemistry, 272(20), 13397-13402 (1997-05-16)
Aggregation of the high affinity IgE receptor (FcepsilonRI) in a mast cell line resulted in activation of the p42 and the stress-activated p38 mitogen-activated protein (MAP) kinases. Selective inhibition of these respective kinases with PD 098059 and SB 203580 indicated
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持