Name: MAPKAP Kinase 2 Protein, active, 10 µg
Brand: MM

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About This Item

UNSPSC代码:

12352202

eCl@ss:

32160405

NACRES:

NA.41

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生物来源

human

质量水平

100

重组

expressed in E. coli Human MAPKAP Kinase
expressed in E. coli

产品线

Upstate^®

比活

(For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)

分子量

M_w 70.2 kDa

纯化方式

(GSH-agarose & Q-Sepharose FF)

种属反应性

human

制造商/商品名称

Upstate^®

技术

activity assay: suitable (kinase)

登记号

(FUNCTION: SwissProt: P49137 # Its physiological substrate seems to be the small heat shock protein (HSP27/HSP25). In vitro can phosphorylate glycogen synthase at ′Ser-7′ and tyrosine hydroxylase (on ′Ser-19′ and ′Ser-40′). This kinase phosphorylates Ser in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue (By similarity). Mediates both ERK and p38 MAPK/MAPK14 dependent neutrophil responses. Participates in TNF alpha-stimulated exocytosis of secretory vesicles in neutrophils. Plays a role in phagocytosis-induced respiratory burst activity. )
NM_004759.3

一般描述

Product Source: Human MAPKAP Kinase 2 expressed in E. coli.

Recombinant human MAPKAP Kinase 2, residues 46-400, A399G, containing an N-terminal GST tag.

MAPKAP Kinase 2 has been implicted as a cell cycle checkpoint kinase, joining the ranks of Chk1 and Chk2 as critical regulators of the DNA damage response in mammalian cells in recent studies (Manke, et al, 2005, and Abraham, 2005). This research indicates that MAPKAP Kinase 2, a serine/threonine kinase activated by p38, phosphorylates CDC25 B/C, generating a binding site for 14-3-3 proteins. The checkpoint kinases Chk1 and Chk2 are targets for drug discovery efforts designed to overcome cell cycle arrest due to DNA damage induced by chemotherapeutic agents. Overriding the checkpoint kinases in cancer cells exposed to DNA damaging agents leads to catastrophic failure of cell division and apoptosis.
The new role of MAPKAP Kinase 2 in complementing the function of Chk1 and Chk2 suggests a new target for drug discovery efforts.

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene.

生化/生理作用

Protein Target: MAPKAP-K2

Target Sub-Family: CAMK

包装

Also available in 10 μg size and in bulk

Also available in 250μg size as catalog # 14-337M - Call for pricing and availability.

质量

Routinely evaluated by phosphorylation of MAPKAP Kinase 2 Substrate Peptide

目标描述

MAPKAP-K2

外形

GSH-agarose & Q-Sepharose FF

储存及稳定性

1 year at -20°C

其他说明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形图

GHS07

警示用语：

Warning

危险声明

H317

预防措施声明

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

危险分类

Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

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