ROKα/ROCK-II Protein, active, rat, 10 µg

N-terminal, His-tagged, fusion protein corresponding to amino acids 2-543 of rat ROKa/ROCK-II.

Note: The preparation contains numerous Sf21 cellular proteins which bind weakly to Ni-NTA agarose.

Product Source: Rat ROKa/ROCK-II expressed in Sf21 insect cells.

Protein Target: ROKα/ROCK-II

Target Sub-Family: AGC

10μg of enzyme in 2.3μl of 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 2mM benzamidine, 0.4mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

Ni-NTA agarose

6 months at -70°C

Rroutinely evaluated using the S6 Kinase Assay Kit

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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