14-383
Rho检测试剂（Rhotekin RBD，琼脂糖），650 µg
Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.
别名:
Rhotekin RBD检测试剂, Rho分析试剂, Rho检测试剂
产品名称
Rho检测试剂（Rhotekin RBD，琼脂糖），650 µg, Specifically binds to & precipitates GTP-Rho, but not GDP-Rho from cell lysates. For use in Affinity Binding Assays.
biological source
mouse
manufacturer/tradename
Upstate®
technique(s)
affinity binding assay: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Gene Information
mouse ... RTKN(20166)
Analysis Note
通过从3T3/A31裂解物中沉淀GTP-Rho进行常规评估，随后使用抗RhoA，克隆55（05-778）通过免疫印迹分析进行检测。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
GST标记的融合蛋白，对应于小鼠Rhotekin Rho结合域的残基7-89，在大肠埃希菌中表达并与谷胱甘肽-琼脂糖结合。特异性结合并沉淀细胞裂解物中的GTP-Rho，但不沉淀GDP-Rho。
产品来源：在大肠埃希菌中表达
Other Notes
比活相关数据，请参阅检验报告以获取该酶的单独批次。
Physical form
650 µg重组蛋白溶于1 mL谷胱甘肽-琼脂糖浆液中，其中包含45mM TRIS（pH 7.5）、0.45%Triton X-100、135mM NaCl，4.5 mM MgCl2、0.9 mM二硫苏糖醇、0.09 mM PMSF、0.9 µg/mL抑肽酶、0.9 µg/mL亮肽素和10%甘油。
Preparation Note
自收到之日起在-70°C下可稳定保存6个月。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
存储类别
10 - Combustible liquids
wgk
WGK 3
Minji Jo et al.
The Journal of biological chemistry, 277(14), 12479-12485 (2002-01-24)
Binding of the urokinase-type plasminogen activator (uPA) to its receptor activates diverse cell signaling pathways. How these signals are integrated so that cell physiology is altered remains unclear. In this study, we demonstrated that migration of MCF-7 breast cancer cells
Simvastatin reduces human atrial myofibroblast proliferation independently of cholesterol lowering via inhibition of RhoA
Porter, K. E., et al
Cardiovascular Research, 61, 745-755 (2004)
Progestogens regulate endothelial actin cytoskeleton and cell movement via the actin-binding protein moesin.
Xiao-Dong Fu, Marina Flamini, Angel Matias Sanchez, Lorenzo Goglia, Maria Silvia Giretti et al.
Molecular Human Reproduction null
Geranylgeranyl diphosphate depletion inhibits breast cancer cell migration.
Amel Dudakovic,Huaxiang Tong,Raymond J Hohl
Investigational New Drugs null
Determination of GTP loading on Rho.
X D Ren et al.
Methods in enzymology, 325, 264-272 (2000-10-19)
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