一般描述
对应于全长绿色荧光蛋白的N末端带有HIS标签的融合蛋白，在大肠埃希菌中表达。绿色荧光蛋白(GFP)是维多利亚多管发光水母(Aequorea Victoria)中的一种细胞质蛋白。
生化/生理作用
绿色荧光蛋白(GFP)可以通过遗传改造，跟许多种蛋白形成融合蛋白，同时保持目标蛋白的活性和GFP的荧光。GFP可用作基因表达的报告基因，也可作为活细胞蛋白质定位研究的融合标签。还作为光学和显微探针，分析各种系统的生物功能。GFP非常稳定，适合作为体外研究工具使用。
外形
含20%甘油的PBS。
制备说明
在-20°C下保存1年
分析说明
常规评估为蛋白凝胶和蛋白质免疫印迹的标准品，用于校准荧光计以及显微注射到细胞和组织中。
法律信息
GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Sonit Kumar Gogoi et al.
Langmuir : the ACS journal of surfaces and colloids, 22(22), 9322-9328 (2006-10-18)
Recombinant Escherichia coli (E. coli) bacteria expressing green fluorescent protein (GFP) was used as a model system to investigate the antimicrobial activities of Ag nanoparticles (NPs). A convenient in situ method of Ag NP synthesis using sodium borohydride, in the
Sebastian Strauss et al.
Nature methods, 15(9), 685-688 (2018-08-22)
Although current implementations of super-resolution microscopy are technically approaching true molecular-scale resolution, this has not translated to imaging of biological specimens, because of the large size of conventional affinity reagents. Here we introduce slow off-rate modified aptamers (SOMAmers) as small
Vincent Jung et al.
Proteomics, 22(9), e2100031-e2100031 (2021-12-28)
Biolayer interferometry (BLI) is a technology which allows to study the affinity between two interacting macro-molecules and to visualize their kinetic of interaction in real time. In this work, we combine BLI interaction measurement with mass spectrometry in order to
Dominique C S Evans et al.
Microbial cell (Graz, Austria), 10(7), 145-156 (2023-07-03)
Staphylococcus aureus is a major human pathogen that utilises many surface-associated and secreted proteins to form biofilms and cause disease. However, our understanding of these processes is limited by challenges of using fluorescent protein reporters in their native environment, because
J L Casey et al.
Protein engineering, 13(6), 445-452 (2000-07-06)
We produced a fluorescent antibody as a single recombinant protein in Escherichia coli by fusing a red-shifted mutant of green fluorescent protein (EGFP) to a single-chain antibody variable fragment (scFv) specific for hepatitis B surface antigen (HepBsAg). GFP is a
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