ReadyPlate^™ Mannitol Egg Yolk Polymyxin Agar

ReadyPlate^™ is ready to use media to provide the highest level of quality and testing confidence for improving accuracy and significantly reducing the risk of false results. ReadyPlate^™ Mannitol Egg Yolk Polymyxin Agar is used for the confirmation and enumeration of colonies of Bacillus cereus.

ReadyPlate^™ Mannitol Egg Yolk Polymyxin Agar is used for the confirmation and enumeration of colonies of Bacillus cereus in food samples.

The MYP agar differentiates B. cereus from other bacteria by its resistance to Polymyxin B, its inability to produce acids from mannitol, and the presence of lecithinase. Peptone and meat extract serve as carbon and nitrogen sources for B. cereus. Bacteria that ferment mannitol to produce acids produce yellow staining of the medium with phenol red as a pH indicator. The lecithin present in egg yolk is cleaved by the lecithinase-producing B. cereus, leading to the formation of an opalescent white precipitation zone surrounding the colonies. Since B. cereus is mannitol-negative, the agar around colonies remains unchanged or is discolored by light alkalization (pink colonies). E. coli, other Enterobacteriaceae, and P. aeruginosa are inhibited by Polymyxin B.

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