Tryptone Bile X-Glucuronide Agar

ReadyPlate^™ Tryptone Bile X-Glucuronide Agar plates are ready-to-use, sterile 90mm plates for the enumeration, detection, and identification of β-glucuronidase positive Escherichia coli from food and feeding stuff. The presence of the enzyme β-D-glucuronidase differentiates most Escherichia coli from other coliforms. E. coli absorbs the chromogenic substrate 5-bromo-4-chloro-3-indolyl-β-D glucuronide (X-β-D-glucuronide). The enzyme β-glucuronidase splits the bond between the chromophore 5-bromo-4-chloro-3-indolyle- and the β-D-glucuronide forming blue to blue-green colored colonies. The growth of accompanying Gram-positive flora is largely inhibited by the use of bile salts and the high incubation temperature of 44°C.

Tryptone Bile X-Glucuronide Agar plates are ready-to-use, sterile 90mm plates for the enumeration, detection, and identification of β-glucuronidase positive Escherichia coli from food and feeding stuff as well as from environmental samples in the area of food production and food handling.

READYPLATE is a trademark of Merck KGaA, Darmstadt, Germany