biological source
human
recombinant
expressed in E. coli
mol wt
Mw 11 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable, western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
General description
在大肠杆菌中生产的重组人组蛋白H4。
Analysis Note
已作为体外酶反应的底物进行了常规评估。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Expression and purification of recombinant human histones.
Tanaka, Yoshinori, et al.
Methods, 33, 3-11 (2004)
Expression, purification, and structural characterization of human histone H4.
Vergani, Laura, et al.
Protein Expression and Purification, 24, 420-428 (2002)
Characterization of nucleosome core particles containing histone proteins made in bacteria.
Luger, K, et al.
Journal of Molecular Biology, 272, 301-311 (1997)
Matthew V Holt et al.
Current protocols, 1(2), e26-e26 (2021-02-04)
Histones are the primary protein component of chromatin and are involved in virtually all DNA-templated processes. Histones are abundantly post-translationally modified by a variety of chromatin-modifying machinery. These post-translational modifications (PTMs) are recognized by a range of "reader" proteins, which
David Shechter et al.
Nature protocols, 2(6), 1445-1457 (2007-06-05)
Histone proteins are the major protein components of chromatin, the physiologically relevant form of the genome (or epigenome) in all eukaryotic cells. Chromatin is the substrate of many biological processes, such as gene regulation and transcription, replication, mitosis and apoptosis.
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