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14-730-M

c-Kit (V560G) Protein, active, 10 µg

Active, N-terminal GST tagged recombinant human c-Kit, amino acids 544 -end, containing the V560G mutation, found in patients with gastrointestinal stromal tumours. For use in Kinase Assays.

别名:

Mast/stem cell growth factor receptor, SCFR, CD117

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Biological source:
human
Mol wt:
Mw 76.6 kDa
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biological source

human

Quality Level

100, 300

mol wt

Mw 76.6 kDa

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable (kinase)

NCBI accession no.

NM_000222.2, NM_001093772.1

UniProt accession no.

P10721

Gene Information

human ... KIT(3815)

General description

N-terminal GST tagged recombinant human c-Kit, amino acids 544 - end, containing the V560G mutation. The V560G mutation has been found in patients with gastrointestinal stromal tumours and has been linked to poor prognosis. This mutation confers increased susceptibility to Gleevec.
Product Source: Human c-Kit (V560G) expressed in Sf21 insect cells.

Physical form

Presented as a frozen solution in 50mM Tris/HCl pH 7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij 35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, and 0.1% 2-mercaptoethanol.

Analysis Note

Routinely evaluated by phosphorylation of 250μM GGMEDIYFEFMGGKKK.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Juxtamembrane mutant V560GKit is more sensitive to Imatinib (STI571) compared with wild-type c-kit whereas the kinase domain mutant D816VKit is resistant.
Frost, Michelle J, et al.
Molecular Cancer Therapeutics, 1, 1115-1124 (2002)
An in vivo tumor model exploiting metabolic response as a biomarker for targeted drug development.
Cullinane C, Dorow DS, Kansara M, Conus N, Binns D, Hicks RJ, Ashman LK, McArthur GA, Thomas DM.
Cancer Research, 65, 9633-9636 (2005)

全球贸易项目编号

货号GTIN
14-73004053252324956