14-749-M
TAO1 Protein, active, 10 µg
Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.
产品名称
TAO1 Protein, active, 10 µg, Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays.
biological source
human
recombinant
expressed in baculovirus infected Sf21 cells
assay
93% (Coomassie blue staining, SDS-PAGE)
specific activity
1179 U/mg
mol wt
Mw 41 kDa
manufacturer/tradename
Chemicon®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Quality Level
Gene Information
human ... TAOK1(57551)
Biochem/physiol Actions
Protein Target: TAO1
Target Sub-Family: STE
Analysis Note
Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO1.
Application
Research Category
Metabolism
Metabolism
Research Sub Category
Cardiovascular Disease
Metabolic Disorders
Cardiovascular Disease
Metabolic Disorders
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327.
Product Source: Expressed by baculovirus in Sf21 cells.
Other Notes
Specific Activity: where one unit of TAO1 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM.
Physical form
10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Ni2+/NTA-agarose
Preparation Note
Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Isolation of TAO1, a protein kinase that activates MEKs in stress-activated protein kinase cascades.
M Hutchison et al.
The Journal of biological chemistry, 273(44), 28625-28632 (1998-10-24)
Several components of the budding yeast pheromone-response pathway are conserved in mammalian mitogen-activated protein (MAP) kinase pathways. Thus, we used degenerate oligonucleotides derived from the sequence of the Saccharomyces cerevisiae protein kinase Ste20p to amplify related sequences from the rat.
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