14-912-M
TGFBR-1 Protein, active, 10 µg
Active, N-terminal GST-tagged, recombinant, human TGFBR1 amino acids 200–end containing a T204D mutation, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose. For use in Kinase Assays.
别名:
TGF-beta receptor type-1, TGFR-1, Transforming growth factor-beta receptor type I, TGF-beta receptor type I, TbetaR-I, TGF-beta type I receptor, Serine/threonine-protein kinase receptor R4, SKR4, Activin receptor-like kinase 5, ALK-5
登录并加购，请在购物车里查看协议价、货期和发货地。
选择规格
变更视图
关于此项目
UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in baculovirus infected Sf21 cells
Mol wt:
Mw 62 kDa
biological source
human
Quality Segment
recombinant
expressed in baculovirus infected Sf21 cells
mol wt
Mw 62 kDa
manufacturer/tradename
Upstate®
technique(s)
activity assay: suitable (kinase)
NCBI accession no.
UniProt accession no.
Gene Information
human ... TGFBR1(7046)
General description
N-terminal GST-tagged, recombinant, human TGFBR1 amino acids 200–end containing a T204D mutation, expressed by baculovirus in Sf21 insect cells. Purified using glutathione agarose.
Product Source: Expressed by baculovirus in Sf21 insect cells.
Transforming growth factor, beta receptor type I (TGF-beta receptor I) forms a heteromeric complex with TGF-beta receptor type II when bound to TGF-beta. This complex transduces the TGF-beta signal from the cell surface to the cytoplasm. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. TGF-beta receptor I is found in all tissues, but is most abundant in placenta and least abundant in brain and heart tissue. TGF-beta receptor I is phosphorylated at basal levels in the absence of ligand binding and is multiply phosphorylated, mainly in the GS region. Defects in TGF-beta receptor I are the cause of Loeys-Dietz syndrome type 1A (LDS1A), also known as Furlong syndrome or Loeys-Dietz aortic aneurysm syndrome (LDAS).
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Biochem/physiol Actions
Protein Target: TGFBR1
Target Sub-Family: TKL
Packaging
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-912M when ordering the 250 µg size.
Physical form
50mM Tris-HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
Glutathione agarose affinity chromatography
Preparation Note
Store -70°C. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.
Analysis Note
Routinely evaluated by phosphorylation of casein.
Other Notes
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.