IKKε Protein, active, 10 µg

IKBKE is a noncanonical I-kappa-B kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. May play a special role in the immune response. Protects cells against DNA damage-induced cell death.

Product Source: N-terminal FLAG and GST-tagged, recombinant, human IKK full length, expressed by baculovirus in Sf21 insect cells.

Research Category
Signaling

Protein Target: IKKe

Target Sub-Family: Misc

Also available in 250μg size as catalog # 14-926M and in bulk as catalog # 14-926-K.

Purified by immunoaffinity chromatography.

50mM Tris/HCl pH7.5, 150mM NaCl, 10mM β-glycerophosphate, 1mM sodium orthovanadate, 1mM NaF, 28% v/v glycerol, 0.03% Brij-35, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol

6 months at -70°C

Routinely evaluated by phosphorylation of casein.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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