STK25, active

Product Source: N-terminal GST-tagged and C-terminal 6His-tagged, recombinant, human STK25 amino acids 1-308, expressed by baculovirus in Sf21 insect cells

STK25 is a member of the germinal centre kinase III (GCK III) subfamily of the sterile 20 superfamily of kinases. The enzyme plays a role in serine-threonine liver kinase B1 (LKB1) signaling pathway to regulate neuronal polarization and morphology of the Golgi apparatus. The protein is translocated from the Golgi apparatus to the nucleus in response to chemical anoxia and plays a role in regulation of cell death. A pseudogene associated with this gene is located on chromosome 18.

Target Sub-Family: STE

Also available in 250μg size as catalog # 14-929M and in bulk as catalog # 14-929-K.

50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol

Purified using glutathione sepharose.

6 months at -70°C

Routinely evaluated by phosphorylation of RLGRDKYKTLRQIRQ

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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