IRE1, active

Product Source: N-terminal 6His-tagged, recombinant, human IRE1 amino acids 465-end expressed by baculovirus in Sf21 insect cells.

Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis.

Also available in 250μg size as catalog # 14-930M and in bulk as catalog # 14-930-K.

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

6 months at -70°C

Routinely evaluated by phosphorylation of MBP.

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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