GCN2 (EIF2AK4) belongs to a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2S1) to downregulate protein synthesis in response to varied cellular stresses.

Product Source: N-terminal GST-tagged, recombinant, human GCN2 full length, expressed by baculovirus in Sf21 insect cells. The recombinant protein contains the amino acid substitution E556G with reference to GenPept NP_001013725. E556G is reported in GenPept CAH10626.

Target Sub-Family: STE

Also available in 250μg size as catalog # 14-934M and in bulk as catalog # 14-934-K.

Purified using glutathione sepharose.

Protein in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.

6 months at -70°C

Routinely evaluated by phosphorylation of peptide RSRSRSRSRSRSRSR

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

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