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Merck
CN

1.50257

Purospher®STAR RP-18 封端(5 µm)

L × I.D. 250 mm x 10 mm LiChroCART HPLC cartridge, manuCART holder required for use

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Purospher® STAR RP-18 endcapped (5 µm) LiChroCART 250-10

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UNSPSC Code:
41115709
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material

stainless steel column

agency

suitable for USP L1

product line

Purospher®

feature

endcapped

packaging

1 ea of

extent of labeling

17% Carbon loading

parameter

250 bar pressure, 65 °C max. temp.

technique(s)

HPLC: suitable (Semi Prep HPLC), LC/MS: suitable, mass spectrometry (MS): suitable (High)

column L × I.D.

25 cm × 10 mm

surface area

330 m2/g

matrix

fully porous particle

matrix active group

C18 bonding phase

particle size

5 μm

pore size

120 Å pore size

operating pH range

1.5-10.5

separation technique

reversed phase

storage temp.

2-30°C

General description

Purospher® STAR RP-18 endcapped, 5 µm HPLC columns are designed for universal use and allow the separation of basic, neutral, and metal chelating compounds with simple mobile phases and excellent peak symmetry. They are based on ultra-pure fully porous silica particles (Type B). These columns offer an outstanding stability from pH 1.5 to 10.5 over a wide temperature range, and suitability for up to 100% aqueous mobile phases. Plus, they demonstrate best all-round retention characteristics, as proven by the Tanaka test. Purospher® STAR RP-18 endcapped, 5 µm LiChroCART 250-10 is a cartridge column which requires a manuCART 10-II cartridge column holder part number 1.51419.0001. The column dimension of 250 mm length x 10 mm I.D. is the optimal choice for Semi Preparative HPLC separation of complex samples.Learn more about Purospher® STAR HPLC and UHPLC ColumnsLearn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection GuideBrochure: A Practical Guide to HPLC - Method Development Guide

Application

Intended to be used in the separation of aflatoxins, aldehydes, alkaloids, antibiotics, catecholamines, explosives, oligonucleotides, esters, fat soluble vitamins, lipids, fatty acids, flavonoids, ketones, nitrosamines, nucleosides, PAHs, PCBs, peptides, pesticides, phenols, phthalates, preservatives, steroids, metabolized steroids, sulfonamides, sweeteners, and water soluble vitamins.

Features and Benefits

  • Ultra-pure silica (99.999%) for excellent peak symmetry
  • High separation efficiency
  • Reproducible results from run-to-run and batch-to-batch
  • Best all-round performance according to Tanaka
  • Outstanding pH stability from pH 1.5 to 10.5
  • No phase collapse when using highly aqueous mobile phases
  • Good suitability for LC-MS applications

Analysis Note

Pressure: ≤ 140 bar
Theoretical Plates (N/m) (Toluene): ≥ 70000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): ≥ 1.5

Legal Information

LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
MANUCART is a trademark of Merck KGaA, Darmstadt, Germany
Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany

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