产品名称
SeQuant® ZIC-HILIC™ 3.5µm,200Å 100 x 2.1 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Level
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
parameter
550 bar pressure, 70 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
10 cm × 2.1 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
SeQuant® ZIC®-HILIC亲水作用色谱柱专用于保留和分离所有类型的极性和亲水性化合物,实现高选择性和重现性的可靠色谱分析。 ZIC®-HILIC填料的硅胶基质键合着强极性永久两性离子。两性离子电荷1:1平衡,使ZIC®-HILIC色谱柱整体呈中性,具有微弱但重要的离子作用,更利于发挥分离选择性。进行ZIC®-HILIC色谱柱的方法开发时,不受pH影响的永久两性离子更方便选择性调整,保证在优化洗脱剂时,只有分析物受到影响(色谱柱不受影响)。色谱柱的200 Å孔径使其成为在亲水作用色谱条件下分离极性大分子的理想选择。250 mm x 2.1 mm内径的色谱柱规格驻留体积小,是使用UHPLC或优化的HPLC仪器分离复杂样品的最佳选择。优越的保留能力和稳健可靠的性能使得SeQuant® HILIC色谱柱在全球无数实验室备受欢迎。比如,美国FDA(美国食药监)已经推荐SeQuant® ZIC®-HILIC色谱柱用于三聚氰胺及相关物质的分析。SeQuant® HILIC色谱柱具有高稳定和低流失的优点,因而十分适合对背景要求较高的LC-MS应用,高背景会导致信号抑制,并干扰定量检测,同时还会更加损害仪器。高稳定性也令这种高效HILIC色谱柱同样适合传统的HPLC分析。详见Supelco HPLC色谱柱系列和方法开发资料:手册:HPLC和UHPLC色谱柱选择指南 手册:HPLC实用指南——方法开发指南
Analysis Note
理论塔板数(N/m)(胞嘧啶): ≥ 90000
对称性(胞嘧啶):0.95 - 1.40
对称性(胞嘧啶):0.95 - 1.40
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany
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商品
This article evaluates different phase chemistries for stationary phases in Hydrophilic Interaction Liquid Chromatography (HILIC) and uses multivariate analysis for classification based on chemical modification.
Liquid chromatography-mass spectrometry metabolite library for metabolomics: Evaluating column suitability using a scoring approach
Diamantidou D, et al.
Journal of Chromatography A, 1690, 463779-463779 (2023)
Dimitra Diamantidou et al.
Journal of chromatography. A, 1690, 463779-463779 (2023-01-22)
Untargeted metabolomic studies require an extensive set of analyte (metabolic) information to be obtained from each analyzed sample. Thus, highly selective, and efficient analytical methodologies together with reversed-phase (RP) or hydrophilic interaction liquid chromatography (HILIC) are usually applied in these
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| 1504470001 | 04027269134297 |