产品名称
SeQuant® ZIC-HILIC™ 3.5µm,200Å 150 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
Quality Segment
agency
suitable for USP L114
product line
SeQuant®
feature
endcapped: no
analyte functional class(es)
antibiotics, sweeteners, water soluble vitamins
analyte chemical class(es)
PFAS, alcohols / fermentation, aliphatic amines, alkaloids, amino acids, aromatic amines, carboxylic acids as methyl esters, esters, glycans, glycols, inorganic ions, monosaccharides, nitrosamines, nucleosides, oligonucleotides, organic phosphates, peptides, pesticides, phospholipids, polysaccharides, small carbohydrates, sugar alcohols, sulfonamides
parameter
500 bar pressure, 70 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
15 cm × 4.6 mm
matrix
fully porous particle
matrix active group
ZIC-HILIC, ZIC-HILIC bonding phase
particle size
3.5 μm
pore size
200 Å pore size
operating pH
2-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
General description
二氧化硅基ZIC®-HILIC吸附剂有键合的固定相,该固定相由高极性永久两性离子组成。1:1 两性离子电荷平衡有利于分析选择性,使得ZIC®-HILIC柱总体呈电中性,具有重要的弱离子相互作用。方法开发过程中不依赖于pH的永久两性离子有利于调节ZIC®-HILIC 柱的选择性,确保洗脱剂优化过程中只有分析物(而不是柱)受到影响。
200 Å的孔径使该柱特别适合于HILIC条件下较大极性分子的分离。
柱尺寸为250 mm 长 x 2.1 mm 内径,是以具有低滞留体积的UHPLC或优化HPLC仪器分离复杂样品的最佳选择。
由于其有利的截留特性和稳定的性能,SeQuant® HILIC 柱备受全球无数实验室的青睐。例如,美国食品药品监督管理局推荐® 采用ZIC®-HILIC 分析三聚氰胺和相关物质。SeQuant®HILIC具有良好的稳定性和低流失率。这些特点令其特别适用于LC-MS应用,在这种应用中高背景噪音会导致信号抑制和对定量测定的干扰,同时增加仪器磨损。高稳定性令这些高效的HILIC柱受到传统HPLC应用的青睐。
了解有关Supelco HPLC 柱系列和方法开发的更多信息:
手册:HPLC和UHPLC柱选择指南
手册:HPLC实用指南- 方法开发指南
Legal Information
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