产品名称
SeQuant® ZIC®-cHILIC 3µm,100Å 50 x 4.6 mm, PEEK-lined stainless steel column, suitable for HPLC
material
PEEK-lined stainless steel column
product line
SeQuant®
feature
endcapped: no
parameter
500 bar pressure, 70 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
5 cm × 4.6 mm
matrix
fully porous particle
matrix active group
ZIC-cHILIC bonding phase
particle size
3 μm
pore size
100 Å pore size
operating pH
1-7.5
separation technique
hydrophilic interaction (HILIC)
storage temp.
no temp limit
Quality Level
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General description
SeQuant® ZIC®-cHILIC is designed for excellent HPLC and LC-MS of polar hydrophilic compounds. This zwitterionic stationary phase with phosphorylcholine functional group provides you with complementary selectivity for easier method development for substances that have been difficult to separate by previous types of HPLC columns operated in reversed-phase or HILIC mode.The column dimension of 50 mm length x 4.6 mm ID is the best choice for the fast separation of polar compounds using a standard HPLC Instrument.SeQuant® ZIC®-cHILIC HPLC columns provide complementary selectivity to optimize separations of polar hydrophilic compounds. The columns carry a novel densely bonded, 1:1 charge-balanced phosphorylcholine functional group. As with all true zwitterionic stationary phases, straightforward and reproducible separations are achieved by hydrophilic partitioning combined with weak ionic interactions.The more accessible positive charges of SeQuant® ZIC®-cHILIC columns enable selectivity tuning in chromatography through altered interaction with anionic and cationic molecular moieties. This can be favorable in several situations, for example in the separation of labile metal complexes or highly polar and multiply charged aminoglycosides. Their extraordinary stability and minimized bleed also make SeQuant® ZIC®-cHILIC columns the preferred choice for many LC-MS applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide
Analysis Note
Pressure: ≤ 20 bar
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25
Theoretical Plates (N/m) (Cytosine): ≥ 90000
Symmetry (Cytosine): 0.95 - 1.25
Legal Information
SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
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