产品名称
LiChrospher® 100 RP-18 endcapped (10 µm) LiChroCART™ 250-4, suitable for HPLC
material
stainless steel column
agency
suitable for USP L1
product line
LiChrospher®
feature
endcapped
packaging
1 ea of
extent of labeling
21.6% Carbon loading
parameter
250 bar pressure, 60 °C max. temp.
technique(s)
HPLC: suitable, mass spectrometry (MS): suitable (Low - Medium)
column L × I.D.
25 cm × 4 mm
surface area
350 m2/g
matrix
fully porous particle
matrix active group
C18 bonding phase
particle size
10 μm
pore size
100 Å pore size
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
Quality Level
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General description
HPLC cartridge
LiChrospher® RP-18 endcapped is a reliable and versatile traditionally produced spherical silica gel carrier with C-18 endcapped reversed phase properties. It is well suited for the separation of neutral, acidic and weak basic compounds. This sorbent is packed into a LiChroCART 250-4 HPLC cartridge. The connection to the HPLC system is being accomplished by use of the manu-CART NT cartrige holder (1.51486).
Application
- Integration of Solid-Phase Extraction and Reversed-Phase Chromatography in Single Protein-Coated Columns for Direct Injection of Bupivacaine in Human Serum: This study highlights the use of the LiChrosorb® RP-8 HPLC column in a novel method integrating solid-phase extraction with reversed-phase chromatography. This approach significantly enhances the efficiency and accuracy of bupivacaine analysis in human serum, underscoring the column′s utility in clinical and pharmaceutical settings. The research demonstrates the column′s high performance in complex sample matrices, making it an invaluable tool for analytical chemists and researchers in drug monitoring and pharmacokinetics (Zarad et al., 2020).
Analysis Note
Pressure: ≤ 40 bar
Theoretical Plates (N/m) (Toluene): ≥ 20000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.5 - 3.0
Theoretical Plates (N/m) (Toluene): ≥ 20000
Symmetry (Toluene): 0.8 - 1.4
Capacity factor (Toluene): 1.5 - 3.0
Legal Information
LICHROCART is a trademark of Merck KGaA, Darmstadt, Germany
LiChrosorb is a registered trademark of Merck KGaA, Darmstadt, Germany
LiChrospher is a registered trademark of Merck KGaA, Darmstadt, Germany
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