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Merck
CN

16-157

蛋白质A琼脂糖/鲑鱼精子DNA,2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

别名:

ChIP agarose beads, ChIP agaraose A beads, ChIP assays

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关于此项目

UNSPSC Code:
12352202
NACRES:
NA.32
eCl@ss:
32160405
Form:
liquid
Biological source:
Staphylococcus aureus
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biological source

Staphylococcus aureus

Quality Segment

form

liquid

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable

suitability

suitable for immunoprecipitation

shipped in

wet ice

General description

与含有经超声处理的鲑鱼精子DNA的琼脂糖通过烷基胺键共价结合的蛋白A。 鲑鱼精子DNA可封闭与染色质IP(ChIP)分析用微珠的非特异性相互作用。请注意ChIP试剂盒中的组分号16-157C不可单独订购,请购买16-157。
来自金黄色葡萄球菌的蛋白A对于IgG分子的Fc区具有非常高的亲和力。将蛋白A固定在微珠上会形成一种亲和树脂,可用于从粗血清、腹水或细胞培养基中纯化IgG组分。

Application

适合用于染色质免疫沉淀(ChIP)分析

Physical form

含有0.05%叠氮化钠的10mM Tris-HCl, 1mM EDTA, pH 8.0溶液。

Analysis Note

在染色质免疫沉淀中通过拉下与乙酰化组蛋白交联的DNA而进行常规评估; 后续的检测是采用ChIPs级(06-866)抗-乙酰化组蛋白H4抗体进行的

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany


存储类别

12 - Non Combustible Liquids

wgk

WGK 1

法规信息

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相关内容

蛋白质是细胞内部的主要功能分子,负责细胞功能和生存的多种生物学活动。值得关注的是,许多蛋白质也能与DNA发生相互作用。

Protein and nucleic acid interaction reagents and resources for investing protein-RNA, protein-DNA, and protein-protein interactions and associated applications.

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全球贸易项目编号

货号GTIN
16-15704053252515330