The apparent molecular weight is related to the tagged protein.

Recombinant proteins containing either an amino or carboxyl-terminal HHHHHHGS epitope tag, which is encoded by most His6 tag expression vectors. NOTE: The [GS] amino acids on the epitope tag are required for antibody recognition.

N-terminal His-tagged and truncated TRAP protein (amino acids 26-248) from P. falciparum expressed in E. coli. (catalog #05-531), clone 4D11.

Anti-Histidine Tagged Antibody (anti His antibody), clone 4D11, biotin conjugate is an antibody against Histidine Tagged for use in WB.

Research Category
Epitope Tags & General Use

Research Sub Category
Epitope Tags

Protein G Purified

PBS, 0.05% sodium azide before the addition of glycerol to 30%

1 year at -20°C

Routinely evaluated in western blot to detect recombinant N-terminal and C-terminal His-tagged proteins

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