一般描述
Immunogen: GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH.
Specificity: Recognizes GST-fusion proteins.
Specificity: Recognizes GST-fusion proteins.
Glutathione S transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunological, and structural properties, the soluble human GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
特异性
Recognizes GST-fusion proteins.
免疫原
GST (Glutathione S-transferase) derived from the pGEX expression vector and linked to KLH. Clone DG122-2A7.
应用
Anti-GST Tag Antibody, clone DG122-2A7, HRP conjugate detects level of GST Tag & has been published & validated for use in WB.
Research Category
Epitope Tags & General Use
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Epitope Tags
质量
Routinely evaluated by western blot Analysis on recombinant GST protein.
Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.
Western Blot Analysis:
1:500-1:1000 dilution of this lot detected recombinant GST protein.
外形
Protein G Purified
Purified mouse monoclonal IgG in buffer containing (HRP) in 70% storage buffer PBS with 0.05% Kathon® and 30% glycerol. Liquid at -20°C.
储存及稳定性
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析说明
Control
Cells expressing the GST-tag.
Cells expressing the GST-tag.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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危险声明
危险分类
Aquatic Chronic 2 - Skin Sens. 1
储存分类代码
10 - Combustible liquids
WGK
WGK 3
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