biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
11E6, monoclonal
species reactivity
rat, canine, human, mouse
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer473)
Quality Level
General description
60 kDa
Immunogen
KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473.
Application
Detect phospho-Akt1/PKBα (Ser473) using this Anti-Akt1 Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Biochem/physiol Actions
Recognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473.
Physical form
Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography
Preparation Note
1 year at 4°C from date of shipment
Analysis Note
Routinely evaluated by immunocytochemistry and flow cytometry.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Alexa Fluor is a registered trademark of Molecular Probes, Inc.
Alexa Fluor is a registered trademark of Molecular Probes, Inc.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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