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Merck
CN

17-10077

Magna ChIP® HT96 Chromatin Immunoprecipitation Kit

The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.

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UNSPSC Code:
12161503
NACRES:
NA.41
eCl@ss:
32161000
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产品名称

Magna ChIP® HT96 Chromatin Immunoprecipitation Kit, The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.

manufacturer/tradename

Magna ChIP®

technique(s)

immunoprecipitation (IP): suitable

shipped in

dry ice

Quality Level

相关类别

Application

Research Category
Epigenetics & Nuclear Function
Used to detect/quantify: Protein A+G

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument.Each kit includes a complete set of validated, quality controlled reagents, plus a detailed protocol.

Features & Benefits
  • Complete set of materials for up to 96 ChIP reactions
  • Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
  • Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
  • Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systems
  • Protocols for using cells or tissues
  • Direct analysis of DNA without additional clean-up steps
  • Compatible with ChIPAb+ validated antibody and primer sets

Other Notes

Magna ChIP protein A/G magnetic beads

HT96 Nuclei Isolation Buffer

HT96 ChIP Buffer (Sonication/ChIP/Wash)

Low Stringency IP Wash Buffer

HT96 ChIP Elution Buffer

Proteinase K Solution

Protease Inhibitor Cocktail III

10X Glycine

10X PBS

96 Well ChIP Plate

96 Well Thermal Plate

Plate Seal

Strip Caps

Packaging

Kit capacity: 96 chromatin immunoprecipitation assays

Physical form

Two boxes containing all necessary reagents to perform 96 chromatin immunoprecipitation reactions in a 96 well plate format. Supplied buffers are sufficient to generate 24 individual chromatin preparation reactions using 15 cm plates of cultured cells or tissue samples.

Preparation Note

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

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Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2 - Skin Irrit. 2

存储类别

10-13 - German Storage Class 10 to 13

wgk

WGK 3

法规信息

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实验方案

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

相关内容

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

New Products: Antibodies, Assays, Small Molecules, Inhibitors, and Proteins

Magna ChIP™ HT96 Kits for high throughput chromatin immunoprecipitation

Superior enrichment, low background. With performance proven for both qPCR and ChIP-seq analysis, the Magna ChIP™ HiSens kit may be the only ChIP kit you’ll ever need. Outperforming any competing kit, this revolutionary approach to ChIP enables enrichment from both low and high amounts of input chromatin while also delivering low backgrounds and high signal-to-noise ratios for ultra-sensitive detection.

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