In Vitro Tubulin Polymerization Assay Kit (≥99% Pure Bovine Tubulin)

EMD Millipore’s In vitro Tubulin Polymerization Assay Kit allows for quick and quantitative determination of tubulin polymerization due to protein and compound-based modulation.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Introduction
Microtubules are long, hollow cylinders made up of polymerised α- and β-tubulin dimers. Tubulin dimers polymerize end to end in protofilaments which are the building block for the microtubule structure. Microtubules are part of a structural network (the cytoskeleton) within the cell′s cytoplasm. The primary role of the microtubule cytoskeleton is mechanical. However, in addition to structural support, microtubules also take part in many other processes. A microtubule is capable of growing and shrinking in order to generate force, and there are also motor proteins that allow organelles and other cellular factors to be carried along a microtubule.

Tubulin is a highly-conserved major component of eukaryotic cells and the ‘building block’ of microtubules. Tubulin is a heterodimer of α and β subunits with an apparent molecular mass of ~110 kDa. In vivo and in vitro, in the presence of GTP, tubulin heterodimers can polymerize (assemble) to form microtubules.

Many proteins or small-molecule compounds affect microtubule assembly. EMD Millipore’s In vitro Tubulin Polymerization Assay Kit allows for quick and quantitative determination of tubulin polymerization due to protein and compound-based modulation.

≥99% Pure Bovine Tubulin: 2 vials of 130 µL (240 µM) each of ≥99% pure bovine tubulin stock solution in 1X polymerization buffer (PB) containing 1mM GTP.

Polymerization Buffer (PB) 5X: One vial containing 300 µL 5X polymerization buffer.

200mM GTP: 1 vial containing 10 µL of 200 mM GTP.

One 96-well half area plate.

Paclitaxel: One vial containing 10 µL of 700 µM paclitaxel in DMSO.

Nocodazole: One vial containing 10 µL of 700 µM nocodazole in DMSO.

Store tubulin stock solution at -80°C, store all other components at -20°C for up to 4 months from date of receipt. Do not freeze and thaw.

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