LentiBrite GFP-LC3-II Enrichment Kit (Flow Cytometry)

Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.

With EMD Millipore, epigenetics technology has never been more accessible or easier to use. From highly specific antibodies, to rapid bisulfite kits, to industry leading ChIP and next generation sequencing kits, we simplify epigenetics applications to allow you to design the most ambitious experiments to interrogate gene regulation. With our legacy of expertise from Chemicon® and Upstate®, we are committed to molding sophisticated yet simple solutions for understanding epigenetic regulation.

Autophagy Signaling Pathways and Detection