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Merck
CN

17-10532

ChIPAb+ PCAF Antibody

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UNSPSC Code:
12352203
NACRES:
NA.84
eCl@ss:
32160702

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antibody form

purified antibody

clone

polyclonal

species reactivity

rabbit, human

technique(s)

ChIP: suitable
western blot: suitable

suitability

suitable for immunoprecipitation

UniProt accession no.

Q92831

Quality Level

100

Analysis Note

Chromatin Immunoprecipitation:
Sonicated chromatin prepared from K562 cells fixed for 10 min (4e6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 0.7 µg of either Normal Rabbit IgG (Part No. PP64B), or 0.7 µg Anti-PCAF (Part No. CS216159) and the Magna ChIP HiSens Kit (Cat. # 17-10460). Successful immunoprecipitation of PCAF associated DNA fragments was verified by qPCR using ChIP Primers, PRAME (Part No. CS216150).
Please refer to the MagnaChIP HiSens (Cat. # 17-10460) or EZ-MagnaChIP HiSens (Cat. # 17-10461) protocol for experimental details.

Application

Epitope: Located between amino acids 782-832

Biochem/physiol Actions

Recognizes PCAF in regions between a.a. 782-832.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Recombinant protein corresponding to human PCAF (a.a. 782-832).
~93 kDa

Other Notes

Demonstrated to react with human. May react with additional species due to sequence homology.
Includes normal rabbit IgG and primers specific for the human PRAME.

Packaging

25 assays per set. Recommended use: 0.7 μg of antibody per chromatin immunoprecipitation (dependent upon biological context).

Physical form

Anti-PCAF (Rabbit Polyclonal), Part No. CS216159. One vial containing 17.5 µg purified rabbit polyclonal in 25 µL buffer containing Tris-citrate/phosphate buffer (pH 7 to 8) and 0.09% sodium azide, before the addition of 30% glycerol (0.7 mg/mL final). Store at -20°C.

Normal Rabbit IgG, Part No. PP64B. One vial containing 125 μg of purified Rabbit IgG in 125 μL storage buffer containing 0.05% sodium azide. Store at -20°C.

ChIP Primers, PRAME. Part No. CS216150. One vial containing 75 μL of 5 μM of each primer specific for human preferentially expressed antigen in melanoma (chr22:22901678+22901804, hg19 build). Store at -20°C.
FOR: GAA GCC TCG CCG GAA CTC
REV: TCC TCC CCA TCT CTG CAG AA

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

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Advance your Epigenetics Research

Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.

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