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Merck
CN

17-603

Sigma-Aldrich

ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set

ascites fluid, from mouse

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.52

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生物来源

mouse

抗体形式

ascites fluid

克隆

monoclonal

种属反应性

mouse, human

制造商/商品名称

ChIPAb+
Upstate®

技术

ChIP: suitable
western blot: suitable

NCBI登记号

NM_000125.2

UniProt登记号

P03372

运输

dry ice

基因信息

human ... ESR1(2099)

相关类别

一般描述

66 kDa
Every lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. ERα antibody is functionally validated in the precipitation of ERα associated chromatin.
The qPCR primers included flank the ERα binding site in human pS2 promoter.

免疫原

The ERα antibody is made against aa 120-170 of bovine estrogen α.

应用

Chromatin Immunoprecipitation:
Sonicated chromatin, prepared from 3x106 MCF7 cells that are either estrogen starved or b-estradiol treated (100 nM, 45 min.) was subjected to chromatin
immunoprecipitation using 4 mL anti-ERa and the Magna ChIP® G (Cat.# 17-611) kit Standard Protocol. Successful enrichment of ER associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures).
Please refer to the EZ-Magna ChIP G (Cat.# 17-409) or EZ-ChIP (Cat.# 17-371) kit protocols for experimental details.

Western Blot Analysis:
MCF7cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-ERa (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
This ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

生化/生理作用

Estrogen Receptor α

包装

25 assays per kit. ~4 μL per chromatin immunoprecipitation.

外形

Anti-ERα (mouse ascites). 1 vial containing 100 mL ascites. Store at -20°C. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα.

ChIP primers TFF1 (pS2). 1 vial containing 75 mL of 5 μM of each control primer specific for human TFF1 (pS2) promoter. Store at -20°C.
FOR: CCG GCC ATC TCT CAC TAT GAA
REV: CCT TCC CGC CAG GGT AAA TAC

制备说明

1 year at -20°C from date of shipment

分析说明

Chromatin immunoprecipitation:
Sonicated chromatin prepared from 3x106 MCF7 cells that were treated with b-estradiol (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERα and beads only control.
Successful enrichment of ERα associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures).
Please refer to the EZ-Magna ChIP G (Cat.# 17- 409) or EZ-ChIP (Cat.# 17-371) kit protocols for experimental details.
Control
Includes control primers specific for human TFF1 (pS2) promoter.

法律信息

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

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L Zeng et al.
Endocrine-related cancer, 23(2), 125-134 (2015-12-10)
Breast cancer patients with diabetes respond less well to chemotherapy; in keeping with this we determined previously that hyperglycaemia-induced chemoresistance in estrogen receptor (ERα) positive breast cancer cells and showed that this was mediated by fatty acid synthase (FASN). More
Chonthicha Satirapod et al.
Aging, 12(8), 7313-7333 (2020-04-18)
Progressive loss of ovarian estrogen (E2) production is a hallmark feature of, if not a driving force behind, reproductive aging and the menopause. Recent genetic studies in mice have shown that female germline or oogonial stem cells (OSCs) contribute to
Lu Gao et al.
The Journal of clinical endocrinology and metabolism, 101(10), 3646-3656 (2016-07-28)
The initiation of term and preterm labor is associated with an up-regulated inflammatory response in myometrium; however, the underlying signaling pathways remain incompletely defined. To define the regulatory mechanisms that mediate the increased myometrial inflammatory response leading to labor, we
Rong-Yi Chen et al.
Journal of immunology (Baltimore, Md. : 1950), 194(8), 4019-4028 (2015-03-15)
The symptoms of vaginal candidiasis exacerbate in the second half of the menstrual cycle in premenopausal women when the serum estradiol level is elevated. Estradiol has been shown to inhibit Th17 differentiation and production of antifungal IL-17 cytokines. However, little
Valentina Vacca et al.
Scientific reports, 6, 18980-18980 (2016-01-09)
Sex differences play a role in pain sensitivity, efficacy of analgesic drugs and prevalence of neuropathic pain, even if the underlying mechanisms are far from being understood. We demonstrate that male and female mice react differently to structural and functional

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