17-622
ChIPAb+ 三甲基-组蛋白H3（Lys27）- ChIP验证的抗体和引物组
from rabbit, purified by using Protein A
别名:
Chip Antibody and primer set, H3K27me3 ChIP, H3K27me3, Histone H3 (tri methyl K27), Histone H3K27me3, Histone H3K27me3 ChIP
生物来源
rabbit
质量水平
克隆
polyclonal
纯化方式
using Protein A
种属反应性
human, mouse
制造商/商品名称
ChIPAb+
Upstate®
技术
ChIP: suitable
western blot: suitable
NCBI登记号
UniProt登记号
运输
dry ice
基因信息
human ... H3F3B(3021)
一般描述
每次对全部ChIPAb+抗体的染色质沉淀进行单独验证。每个ChIPAb+抗体组均包含对照引物（通过qPCR对每个批次进行检测），以在基因座特异性环境下对IP结果进行生物学验证。提供了qPCR方案和引物序列，使研究人员能够在染色质环境中使用我们的抗体时验证ChIP方案。
每组还包括阴性对照抗体，以确保ChIP反应的特异性。ChIPAb+三甲基组蛋白H3（Lys27）组包括抗三甲基组蛋白H3（Lys27）抗体，阴性对照抗体（纯化的兔IgG）和qPCR引物，可扩增人α卫星的139 bp区域。三甲基组蛋白H3（Lys27）和阴性对照抗体以可扩展的“每个ChIP”反应大小提供，可用于功能上验证三甲基组蛋白H3（Lys27）相关染色质的沉淀。
免疫原
应用
使用4 μg纯化的抗三甲基组蛋白H3（Lys27）抗体或正常兔IgG和Magna ChIP A试剂盒（目录号17-610）对从2x106 HeLa细胞制备的超声染色质进行染色质免疫沉淀。使用位于人GAPDH启动子侧翼的ChIP引物GAPDH和靶向人MyoD启动子的引物，通过qPCR验证了三甲基组蛋白H3（Lys27）相关DNA片段的成功富集。
关于实验详细信息，请参阅EZ-Magna ChIP A（目录号17-408）或EZChIP（目录号17-371）试剂盒方案。
蛋白质印迹分析：
重组组蛋白H3（泳道2）和HeLa细胞酸提取物（泳道1）通过电泳分离，转移到硝酸纤维素中，用1:5000稀释的抗三甲基组蛋白H3 Lys27（0.4 μg/mL）进行探测。
使用HRP偶联的山羊抗兔二抗和化学发光检测系统观察蛋白质（请参见图）。
组蛋白
表观遗传学&核功能
生化/生理作用
包装
外形
正常兔IgG。一瓶含125 μg纯化兔IgG，溶于125 μL储存缓冲液（含0.1%叠氮化钠）。储存于-20°C。
ChIP引物人α-卫星。一个小瓶含有75 μL 5 μM人α卫星特异性的对照引物。储存于-20°C。
对于：CTG CAC TAC CTG AAG AGG AC
REV: GAT GGT TCA ACA CTC TTA CA
制备说明
分析说明
1小瓶，含有75 μL的5 μM人α卫星特异性对照引物。储存于-20°C。
用于：CTG CAC TAC CTG AAG AGG AC
REV: GAT GGT TCA ACA CTC TTA CA
使用4 μg纯化抗体或正常兔IgG和Magna ChIP® A试剂盒（目录号17-610）对从3x106 NIH3T3 L1细胞制备的超声染色质进行染色质免疫沉淀。使用ChIP引物人α-卫星通过qPCR验证了与三甲基组蛋白H3
（Lys27）相关的DNA片段的成功富集（请参见图）。
关于实验详细信息，请参阅EZ-Magna ChIP A（目录号17-408）或EZ-ChIP（目录号17-371）试剂盒方案。
其他说明
多克隆对照兔血清，1小瓶
人α-卫星引物组，1小瓶
法律信息
免责声明
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
相关内容
Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms. Epigenetic regulation allows a cell to vary its response based on its biological and environmental contexts. Epigenetic changes can effect transcriptional and post-transcriptional regulation via mechanisms such as histone modification, chromatin and nucleosome remodeling, DNA methylation, and small and non-coding RNA-mediated regulation. These mechanisms, in cooperation with transcription factors and other nucleic acid-binding proteins, regulate gene expression. Epigenetic mechanisms of gene regulation impacts diverse areas of research—from agriculture to human health. Common epigenetic assays such as chromatin immunoprecipitation (ChIP) and RNA immunoprecipitation (RIP) rely on high quality antibodies that recognize specific epigenetic modifications for accurate results. EMD Millipore offers over 100 ChIPAb+™ and RIPAb+™ validated antibody kits that are quality tested on ChIP/RIP assays and are conveniently provided with control qPCR primers and negative control antibodies to ensure first time ChIP/RIP success.
Technical Guide to Chromatin Immunoprecipitation: Critical Factors for Success
Genome-wide mapping of DNA-protein interactions and epigenetic marks using chromatin immunoprecipitation combined with next generation sequencing (ChIP-Seq) is indispensable for many gene regulation studies. The Magna ChIPSeq Kit simplifies this technique, enabling the performance of ChIP-Seq by virtually any laboratory.
"Epigenetics describes heritable changes in gene expression caused by non-genetic mechanisms instead of by alterations in DNA sequence. These changes can be cell- or tissue-specific, and can be passed on to multiple generations. Epigenetic regulation enriches DNAbased information, allowing a cell to vary its response across diverse biological and environmental contexts. Although epigenetic mechanisms are primarily centered in the nucleus, these mechanisms can be induced by environmental signals such as hormones, nutrients, stress, and cellular damage, pointing to the involvement of cytoplasmic and extracellular factors in epigenetic regulation."
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