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Merck
CN

189490

3-MA

≥95% (HPLC), powder, autophagy inhibitor, Calbiochem®

别名:

自噬抑制剂，3-MA, 3-甲基腺嘌呤

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关于此项目

经验公式（希尔记法）:
C6H7N5
化学文摘社编号:
5142-23-4
分子量:
149.15
MDL编号:
MFCD00010531
UNSPSC代码:
12352200
NACRES:
NA.77

主要文件

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产品名称

自噬抑制剂，3-MA, Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker. Acts as an inhibitor of III PI3-Kinase.

质量水平

100

方案

≥95% (HPLC)

表单

powder

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
protect from light

颜色

white to faint yellow

溶解性

DMSO: 10 mg/mL
water: 5 mg/mL

运输

ambient

储存温度

2-8°C

SMILES字符串

N1(C2=NC=NC2=C(N=C1)N)C

InChI

1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

InChI key

FSASIHFSFGAIJM-UHFFFAOYSA-N

相关类别

一般描述

一种广泛使用的细胞渗透性自噬隔离阻滞剂，可有效保护小脑颗粒细胞免于血清/钾缺乏后的凋亡。尽管也被成为III类PI3K抑制剂（在无细胞酶法测定中IC50 = 4.5 mM），3-MA展现出了与其他两种PI3K抑制剂显著不同的药理属性，即LY 294002（货号440202和440204）以及Wortmannin（货号681675）

包装

用惰性气体包装

制备说明

在重悬后分装并冻存于冰箱（-20°C。储备溶液在-20°C下可稳定保存至多3个月。
在需要高工作浓度（例如10 mM）的应用中，使用预制的储备液可能会导致将比所需溶剂（例如DMSO）高的溶剂引入实验体系。在这种情况下，建议在将要使用时通过称量固体化合物并直接重新配制成用于实验系统的溶液（缓冲液、培养基等）。可能需要涡旋以实现在水中的完全溶解。

其他说明

Canu, N., et al. 2005.J. Neurochem.92, 1228.
Hirosako, K., et al. 2004.Biochem.Biophys.Res. Commun.316, 845.
Beugnet, A., et al. 2003.生物化学。J.372, 555.
Eskelinen, E.L., et al. 2002.Traffic3, 878.
Petiot, A., et al. 2000.J. Biol. Chem.275, 992.
Seglen, P.O. and Gordon, P.B., 1982.Proc.Natl.Acad.Sci. USA79, 1889.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

毒性：标准处理（A）

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Cong Chen et al.
Cell death & disease, 13(2), 150-150 (2022-02-16)
Ferroptosis, which is characterized by intracellular iron accumulation and lipid peroxidation, is a newly described form of regulated cell death that may play a key role in tumour suppression. In the present study, we investigated the expression profiles and biological
Jinghui Zhai et al.
Journal of biochemical and molecular toxicology, 35(11), e22896-e22896 (2021-08-24)
The NOD-like receptor family pyrin domain-containing (NLRP3) inflammasomes is centrally implicated in cisplatin (CP)-induced kidney injury. Autophagy is critical for inhibiting production of NLRP3 protein that effectively reduces the inflammatory response. Ginsenoside Rg3 (SY), an active component extracted from ginseng
Wei Huang et al.
Frontiers in neuroscience, 15, 745815-745815 (2021-12-07)
Parkinson's disease is a neurodegenerative disorder with an inflammatory response as the core pathogenic mechanism. Previous human genetics findings support the view that the loss of TREM2 function will aggravate neurodegeneration, and TREM2 is one of the most highly expressed
Halima Alsamri et al.
Frontiers in oncology, 12, 911615-911615 (2022-06-18)
We recently reported that carnosol induces ROS-dependent autophagy and apoptosis in breast cancer cells. We also reported that carnosol inhibits breast cancer cell migration, invasion, and in ovo tumor growth, as well as targets STAT3, PCAF, and p300 to proteasome
Nienke Visser et al.
International journal of molecular sciences, 22(5) (2021-03-04)
Elevated activation of the autophagy pathway is currently thought to be one of the survival mechanisms allowing therapy-resistant cancer cells to escape elimination, including for cytarabine (AraC)-resistant acute myeloid leukemia (AML) patients. Consequently, the use of autophagy inhibitors such as
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