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Merck
CN

189738

Streptavidin, TEXAS RED® Conjugate

This Streptavidin, TEXAS RED® Conjugate is validated for use in ELISA, Flow Cytometry, Immunohistochemistry.

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关于此项目

NACRES:
NA.47
UNSPSC Code:
41116133
Clone:
-
Species reactivity:
-
Application:
Citations:
-
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biological source

(Streptomyces avidinii)

form

liquid

contains

≤0.1% sodium azide as preservative

manufacturer/tradename

Calbiochem®

storage condition

do not freeze, protect from light

dilution

(ELISA (1:2000-1:5000)
Flow Cytometry (1:50-1:200)
Immunohistochemistry (1:50-1:200))

shipped in

ambient

Quality Level

General description

Streptavidin conjugated to TEXAS RED® fluorescent dye. Specific for biotin, biotinylated proteins, or biotinylated antibodies.

Application

ELISA (1:2000-1:5000)

Flow Cytometry (1:50-1:200)

Immunohistochemistry (1:50-1:200)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM bicarbonate-borate buffer, 0.9% NaCl, 5 mg/ml BSA, pH 8.1.

Preparation Note

Do not dilute the entire sample at one time. Withdraw aliquots as needed with a micropipette and dilute with 1% solution of BSA in 50 mM sodium phosphate buffer, pH 7.4 and 0.05% sodium azide.

Other Notes

Biotin activity, absorbance ratio, and label:protein molar ratio are determined on a lot-specific basis. Use 1 to 3 µg of the conjugate per 1 x 106 cells suspended in a volume of 0.1 ml. Variables associated with assay conditions will dictate the proper working dilution.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Texas Red is a registered trademark of Life Technologies

Disclaimer

Toxicity: Standard Handling (A)

hcodes

Hazard Classifications

Aquatic Chronic 3

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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