AZOCOLL Substrate, <50 Mesh

General proteolytic substrate. Provides a rapid and simple spectrophotometric method to detect the presence of proteases in aqueous solutions. As the insoluble substrate is cleaved by proteases, it releases a red dye into the surrounding buffer. Incubation of 50 mg AZOCOLL Substrate with 2 units of PRONASE Protease for 15 min yields an absorbance of greater than 1.0 at 520 nm.

This product has been discontinued.


General proteolytic substrate. Provides a rapid, simple method for the detection of proteases in aqueous solutions. Color yield (520 nm): >1.0; blank (520 nm, 50 mg/5 ml buffer, 10 minutes at 37°C, pH 7.0): <0.2. Mesh: <50 (minimum 80%).

Poilane, I., et al. 1998. Can. J. Microbiol. 44, 157.
Braganza, V.J., and Simmons, W.H. 1991. Biochemistry 30, 4997.
Moore, G.L. 1969. Anal. Biochem. 32, 122.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)