208748
Calpain-1 Substrate, Fluorogenic
An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.
别名:
Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH
质量水平
方案
≥95% (HPLC)
表单
lyophilized solid
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
desiccated (hygroscopic)
protect from light
颜色
red
溶解性
DMSO: 1 mg/mL
荧光
λex ~490 nm
λem ~518 nm
运输
wet ice
储存温度
−20°C
相关类别
一般描述
An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (Km = 4.6 µM; kcat = 11 s-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.
生化/生理作用
Cell permeable: no
Primary Target
A sensitive and specific substrate for calpain-1
A sensitive and specific substrate for calpain-1
Product does not compete with ATP.
Reversible: no
包装
Packaged under inert gas
外形
Supplied as a trifluoroacetate salt.
制备说明
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH
Mittoo, S., et al. 2003. Anal. Biochem.319, 234.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
David S Dang et al.
Meat science, 162, 108039-108039 (2020-01-15)
The purpose of this study was to examine the role of mitochondria in postmortem calcium homeostasis and its effect on proteolysis and tenderness. We hypothesized that mitochondria buffer cytosolic calcium levels and delay the activation of calpain-1 and subsequently the
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持