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208748

Calpain-1 Substrate, Fluorogenic

Name: Calpain-1 Substrate, Fluorogenic
Brand: MM

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin.

别名:

Calpain-1 Substrate, Fluorogenic, μ-Calpain Substrate, Fluorogenic, H-K(FAM)-EVY~GMMK(DABCYL)-OH

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关于此项目

经验公式（希尔记法）:

C₇₉H₉₅N₁₃O₁₉S₂

分子量:

1594.81

NACRES:

NA.77

UNSPSC Code:

12352200

Assay:

≥95% (HPLC)

Form:

lyophilized solid

Quality level:

100

Storage condition:

OK to freeze, desiccated (hygroscopic), protect from light

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属性

Quality Level

100

assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem^®

storage condition

OK to freeze, desiccated (hygroscopic), protect from light

color

red

solubility

DMSO: 1 mg/mL

fluorescence

λ_ex ~490 nm, λ_em ~518 nm

shipped in

wet ice

storage temp.

−20°C

说明

General description

An internally quenched fluorogenic substrate peptide derived from the calpain-1 cleavage site of α-spectrin. It is not recognized by trypsin or α-chymotrypsin and serves as a sensitive and specific substrate for calpain-1 (K_m = 4.6 µM; k_cat = 11 s^-1). Cleavage occurs at the Tyr-Gly bond and results in enhanced fluorescence. Excitation max: ~490 nm; emission max: ~518 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
A sensitive and specific substrate for calpain-1

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Physical form

Supplied as a trifluoroacetate salt.

Preparation Note

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

H-Lys(FAM)-Glu-Val-Tyr~Gly-Met-Met-Lys(DABCYL)-OH

Mittoo, S., et al. 2003. Anal. Biochem.319, 234.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c