assay
>98% (SDS-PAGE)
Quality Segment
form
lyophilized
specific activity
≥35,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
solubility
water: 1 mg/mL
shipped in
wet ice
storage temp.
−20°C
General description
Calmodulin (CaM) purified from porcine brain and biotinylated using NHS ester-activated biotin with an 8 atom spacer arm. A ubiquitous, calcium-binding protein that interacts with and regulates a wide variety of proteins, thus affecting different cellular functions and calcium-calmodulin-dependent signaling pathways. CaM mediates several biological processes, such as inflammation, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth, and the immune response. Useful for enzyme activation, CaM binding studies, and general assessment of CaM-dependent signaling pathways. Biotin/Protein Ratio: 0.5-1.0 as determined by HABA assay.
Physical form
Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Other Notes
Billingsley, M.L., et al. 1985. Proc. Natl. Acad. Sci. USA82, 7585.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
存储类别
10-13 - German Storage Class 10 to 13