产品名称
Anti-Calreticulin Mouse mAb (FMC75), liquid, clone FMC75, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
FMC75, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, monkey
should not react with
rat, E. coli, canine, hamster, salmon, bovine, mouse, guinea pig
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(Enzyme Immunoassay (0.1 µg/mL)
Immunoblotting (0.5 µg/mL)
Immunocytochemistry
Immunohistochemistry
Flow Cytometry )
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Analysis Note
Positive Control
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells
Application
Enzyme Immunoassay (0.1 µg/ml)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Immunoblotting (0.5 µg/ml, colorimetric; see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Flow Cytometry (see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Anti-Calreticulin, mouse monoclonal, clone FMC75, recognizes the ~63 kDa calreticulin in Molt 4, MCF-7, HeLa, K562, A431 & other cells. It is validated for FC, WB, ICC, IHC, and in enzyme immunoassay.
Protein G purified mouse monoclonal antibody. Recognizes the ~63 kDa (apparent MW) calreticulin protein.
Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
Immunogen
Human
a recombinant protein consisting of human calreticulin fused to maltose binding protein
Other Notes
Does not cross-react with GrpE or Grp78. Variables associated with assay conditions will dictate the proper working dilution.
Nauseef, W.M., et al. 1995. J. Biol. Chem. 270, 4741.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Jethmalani, S.M., et al. 1994. J. Biol. Chem. 269, 23603.
Smith, M.J., and Koch, G.L.E. 1989. EMBO J.8, 3581.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 50% Glycerol and 0.09% Sodium Azide.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Stephanie S Lam et al.
Nature methods, 12(1), 51-54 (2014-11-25)
APEX is an engineered peroxidase that functions as an electron microscopy tag and a promiscuous labeling enzyme for live-cell proteomics. Because limited sensitivity precludes applications requiring low APEX expression, we used yeast-display evolution to improve its catalytic efficiency. APEX2 is
Mutation-Driven S100A8 Overexpression Confers Aberrant Phenotypes in Type 1 CALR-Mutated MPN.
Wang, et al.
International Journal of Molecular Sciences, 24 (2023)
Ying Yin et al.
PLoS biology, 16(8), e2005140-e2005140 (2018-08-08)
Some secreted proteins that assemble into large complexes, such as extracellular matrices or hormones and enzymes in storage granules, must be kept at subaggregation concentrations during intracellular trafficking. We show surfeit locus protein 4 (Surf4) is the cargo receptor that
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